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首页> 外文期刊>Separation and Purification Technology >Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt
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Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt

机译：通过添加中性盐在水性两相系统中选择性分配质粒DNA和RNA

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摘要

The selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPSs) by addition of NaCl salt was studied with pure pDNA and RNA solutions. The pDNA is increasingly excluded from top phases upon the addition of 0.5% and 3% (w/w) NaCl. With 3% (w/w) NaCl, the logarithmic partition coefficient of RNA was 1.2 and as a result, the RNA concentration in the top phase was 3.3-fold higher than that in the bottom phase. It is demonstrated that 47%, 13.7% and 7.5% (w/v) of PEG were required to achieve identical precipitation effects with PEG 300,1450 and 6000, respectively. The precipitation efficiency of 6.3% (w/v) PEG 300 corresponds to that of 1% (w/v) PEG 6000. The excluded volume effects in the top phase were probably responsible for the selective exclusion of different nucleic acids species. The results obtained in this study contribute to the basic knowledge of partition of macromolecules in ATPSs in terms of excluded volume theory.
机译：用纯pDNA和RNA溶液研究了通过添加NaCl盐在聚乙二醇（PEG）和磷酸氢二钾双水相两相系统（ATPSs）中对质粒DNA（pDNA）和RNA的选择性分配。通过添加0.5％和3％（w / w）的NaCl，pDNA越来越多地从顶层分离。在3％（w / w）的NaCl中，RNA的对数分配系数为1.2，结果，上层相中的RNA浓度比下层相中的RNA浓度高3.3倍。结果表明，分别要获得与PEG 300、1450和6000相同的沉淀效果，就需要47％，13.7％和7.5％（w / v）的PEG。 6.3％（w / v）PEG 300的沉淀效率相当于1％（w / v）PEG 6000的沉淀效率。顶相中排除的体积效应可能是选择性排除不同核酸种类的原因。从排除体积理论的角度来看，本研究获得的结果有助于ATPS中大分子分配的基础知识。

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