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首页> 外文期刊>Separation and Purification Technology >Two-step Centrifugal Partition Chromatography (CPC) fractionation of Butea monosperma (Lam.) biomarkers
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Two-step Centrifugal Partition Chromatography (CPC) fractionation of Butea monosperma (Lam.) biomarkers

机译：两步离心分离色谱（CPC）分离丁香精油（Lam。）生物标志物

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摘要

Butea monosperma (Lam.) Taubert (Synonym Butea frondosa; family Fabaceae) is a popular plant used in traditional medicine in the Indian continent. The brightly orange flowers of this plant are described with antioxidant potential. The aim of this study was to develop a bio-guided fractionation methodology in order to identify antioxidant biomarkers from B. monosperma flowers which could be used to ensure quality and efficiency of cosmetic products. The hydro-alcoholic extract of B. monosperma flowers was separated using a double Centrifugal Partition Chromatography (CPC) procedure. Presence and/or absence of specific molecule families were evaluated by High Performance Thin Layer Chromatography (HPTLC). The antioxidant activity was performed on each fraction to guide fractionation step. First CPC step separated the crude extract in three fractions of different polarities. The aqueous antioxidant fraction was then subjected to a second CPC separation leading to five sub-fractions. Butrin and isobutrin were isolated from the antioxidant sub-fraction and were highlighted as potential antioxidant marker in B. monosperma flowers. Their chemical structures were confirmed by UV, MS and NMR analyses.
机译：牛油毛（Tubebert monosperma）（Lau。Taubert）（别名牛油菌（Butea frondosa）; Fabaceae家族）是印度大陆上传统医学中常用的一种植物。该植物的鲜艳橙色花朵具有抗氧化潜力。这项研究的目的是开发一种生物引导的分馏方法，以便从单子叶植物的花朵中鉴定抗氧化剂生物标志物，该标志物可用于确保化妆品的质量和效率。使用双离心分配色谱（CPC）程序分离单子叶植物B. monosperma花的水醇提取物。通过高效薄层色谱法（HPTLC）评估特定分子家族的存在与否。在每个馏分上进行抗氧化剂活性以指导分离步骤。 CPC的第一步是将粗提物分为三个极性不同的馏分。然后使含水抗氧化剂级分进行第二次CPC分离，得到五个亚级分。从抗氧化剂子级分中分离出Butrin和isobutrin，并将其突出显示为B. monosperma花中的潜在抗氧化剂标记。通过UV，MS和NMR分析确认了它们的化学结构。

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