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首页> 外文期刊>Separation and Purification Technology >Purification and quantification of ginsenoside Rb3 and Rc from crude extracts of caudexes and leaves of Panax notoginseng
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Purification and quantification of ginsenoside Rb3 and Rc from crude extracts of caudexes and leaves of Panax notoginseng

机译：人参三叶茎叶粗提物中人参皂苷Rb3和Rc的纯化与定量

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In this work,the separation of ginsenoside Rb3 and Rc from the crude extracts of caudexes and leaves of Panax notoginseng(CECLPN)was studied with reversed-phase high-performance liquid chromatography(RP-HPLC).The chromatographic separation was achieved using a SynChropak C18 column(at 25 deg C).A satisfied separation of the compounds was obtained in less than 40min with a flow rate of l.Oml/min.Chromatography was performed using ternary mobile phase composed of methanol,water and phosphoric acid,65:33:1.4(v/v).UV detection was accomplished at 203 nm.Ginsenoside Rb3 and Rc found in the CECLPN constitute up to 38.25% and 26.65% of the dry powder,respectively.Ginsenoside Rb3 and Rc were prepared by semi-preparative HPLC/ELSD using gradient elution system of methanol-water=70:30 ->65:35 at a flow rate of 30 ml/min with a sample load of 300-400 mg.With this chromatographic condition,each individual ginsenoside Rb3 and Rc in purity of more than 97% were gained.The products were confirmed by spectroscopic(UV,IR,ESI-MS/MS,NMR)and HPLC methods.The methods were validated for linearity,precision,recovery,limits of detection(LOD)and limits of quantification(LOQ).The results show that the proposed method appears to be an adequate method for quality control of CECLPN and a useful tool for pharmaceutical study of ginsenosides.
机译：本文采用反相高效液相色谱（RP-HPLC）法研究了从三七人参和叶片粗提物中提取人参皂苷Rb3和Rc的方法。 C18柱（在25摄氏度下），在不到40分钟的时间内以1.0 ml / min的流速获得了令人满意的化合物分离结果。色谱法使用由甲醇，水和磷酸组成的三元流动相进行色谱分离：65： 33：1.4（v / v）。在203 nm处完成紫外检测.CECLPN中发现的人参皂甙Rb3和Rc分别占干粉的38.25％和26.65％。 HPLC / ELSD使用甲醇-水= 70：30-> 65:35的梯度洗脱系统，流速为30 ml / min，样品负载为300-400 mg。在这种色谱条件下，每个人参皂甙Rb3和Rc获得的纯度超过97％。光谱（UV，IR，ESI-MS / MS，NMR）和HPLC方法。方法经线性，精密度，回收率，检测限（LOD）和定量限（LOQ）验证。结果表明，该方法似乎是CECLPN质量控制的适当方法，也是人参皂苷药物研究的有用工具。

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