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首页> 外文期刊>Separation and Purification Technology >Application of response surface methodology to optimize microwave-assisted extraction of silymarin from milk thistle seeds
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Application of response surface methodology to optimize microwave-assisted extraction of silymarin from milk thistle seeds

机译：应用响应面法优化微波辅助从水飞蓟种子中提取水飞蓟素

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摘要

Milk thistle (Silybum marianum L.) is an excellent source of silymarin used as an antioxidant. Microwave-assisted extraction (MAE) was employed to extract silymarin from milk thistle seeds. The effects of four independents variables in terms of extraction time, temperature, ethanol concentration, and solid-liquid ratio on the silymarin yield were determined and the optimal conditions for silymarin were evaluated by means of response surface methodology. Correlation analysis of the mathematical regression model indicated that a quadratic polynomial model could be employed to characterize MAE process for the silymarin. Response surface plots showed that these independent variables, except for extraction time, and interactions significantly influenced the extraction yield of silymarin. The optimal extraction parameters to obtain the highest silymarin yield were time duration of 60 min, temperature of 112 °C, ethanol concentration of 81.5% (v/v), and a solid-liquid ratio of 1:38 (g/mL). The average experimental silymarin yield under the optimum conditions was found to be 56.67 ±1.36 mg/g, which agree with the predicted value of 57.40 mg/g. MAE method was applied successfully to extract silymarin from milk thistle seeds.
机译：蓟（Silybum marianum L.）是水飞蓟素用作抗氧化剂的极好来源。微波辅助提取（MAE）用于从水飞蓟种子中提取水飞蓟素。确定了四个独立变量在提取时间，温度，乙醇浓度和固液比方面对水飞蓟素产量的影响，并通过响应面法评估了水飞蓟素的最佳条件。数学回归模型的相关分析表明，可以使用二次多项式模型来表征水飞蓟素的MAE过程。响应面图表明，这些独立变量，除了提取时间外，以及相互作用都显着影响水飞蓟素的提取率。获得最高水飞蓟素产量的最佳提取参数为：持续时间为60分钟，温度为112°C，乙醇浓度为81.5％（v / v），固液比为1:38（g / mL）。在最佳条件下，水飞蓟素的平均实验产量为56.67±1.36 mg / g，与预测值57.40 mg / g相符。 MAE方法已成功应用于从水飞蓟种子中提取水飞蓟素的研究。

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