Primary capture of high molecular weight nucleic acids using aqueous two-phase systems

Frank Luechau; Tau Chuan Ling; Andrew Lyddiatt

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Primary capture of high molecular weight nucleic acids using aqueous two-phase systems

机译：使用水性两相系统初步捕获高分子量核酸

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The practical feasibility and generic applicability of the selective partition of plasmid DNA(pDNA)and RNA in polyethylene glycol 300(PEG-300)and di-potassium hydrogen phosphate aqueous two-phase systems(ATPS)by addition of NaCl salt was investigated with partially disrupted Escherichia coli(E.coli)cell paste.The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed.The results gained here led the way to an alternative process options for the processing of pDNA.A reproducible process involving two extraction steps to recover pDNA from partially disrupted E.coli cell paste was developed and is presented here.

机译：通过部分加入NaCl盐，研究了质粒DNA（pDNA）和RNA在聚乙二醇300（PEG-300）和磷酸二氢氢钾水溶液两相体系（ATPS）中选择性分配的实际可行性和通用性。该过程产生了相对无蛋白的pDNA溶液，pDNA回收率为89.5％，同时部分去除了低分子量RNA。此处获得的结果为处理提供了另一种工艺选择开发了一种可重复的过程，该过程涉及两个提取步骤，以从部分破坏的大肠杆菌细胞糊中回收pDNA。

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《Separation and Purification Technology》 |2009年第1期|共6页
作者
Frank Luechau; Tau Chuan Ling; Andrew Lyddiatt;
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正文语种 eng
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关键词
Selective partition; Plasmid DNA; ATPS; NaCl; E.coli cell paste;

机译：选择性分配;质粒DNA;ATPS;NaCl;大肠杆菌细胞糊;

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1. Primary capture of high molecular weight nucleic acids using aqueous two-phase systems [J] . Frank Luechau, Tau Chuan Ling, Andrew Lyddiatt Separation and Purification Technology . 2009,第1期

机译：使用水性两相系统初步捕获高分子量核酸
2. Aqueous two-phase poly(ethylene glycol)-poly(acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature [J] . Settu Saravanan, Jonnalagadda Raghava Rao, Balachandran Unni Nair, Process Biochemistry . 2008,第9期

机译：用于蛋白质分配的水相两相聚乙二醇-聚丙烯酸体系：分子量，pH和温度的影响
3. Aqueous-aqueous two-phase systems composed of low molecular weight of polyethylene glycols and dextrans for counter-current chromatographic purification of proteins [J] . Shibusawa Y, Takeuchi N, Sugawara K, Journal of chromatography, B. Analytical technologies in the biomedical and life sciences . 2006,第2期

机译：低分子量聚乙二醇和葡聚糖组成的水-水两相系统，用于蛋白质的逆流色谱纯化
4. The Research for the Extraction of Yeast'S Nucleic Acid with BMIM BF_4-H_2O-KH_2PO_4 Ionic Liquid Aqueous Two-Phase System [C] . Huang Dejuan, Huang Dechao SSITE International Conference on Future Material Research and Industry Application . 2012

机译：用BMIM BF_4-H_2O-KH_2PO_4离子液体水性两相系统提取酵母核酸的研究
5. Molecular Recognition and Structural Influences on Function in Bio-nanosystems of Nucleic Acids and Proteins. [D] . Sethaphong, Latsavongsakda. 2013

机译：分子识别和结构对核酸和蛋白质生物纳米系统功能的影响。
6. Counter-Current Chromatographic Separation of Nucleic Acid Constituents with a Polar Volatile Organic-Aqueous Two-Phase Solvent Systems with ELSD Detection [O] . Yoichi Shibusawa, Go Morikawa, Akio Yanagida, -1

机译：用ELSD检测与极性挥发性有机 - 水性两相溶剂系统的核酸成分的反电流色谱分离
7. Chemical action of X-rays on nucleic acids and related substances in aqueous systems. 2. The mechanism of the action of X-rays on nucleic acids in aqueous systems [O] . Scholes, G., Weiss, J. 1954

机译：X射线对水性系统中核酸和相关物质的化学作用。 2. X射线对水性系统中核酸的作用机理

Primary capture of high molecular weight nucleic acids using aqueous two-phase systems

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