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Continuous fractionation of plasma proteins HSA and HIgG using cascade ultrafiltration systems

机译：使用级联超滤系统连续分离血浆蛋白HSA和HIgG

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摘要

This paper examines the use of multi-stage or cascade ultrafiltration systems for continuous fractionation of model proteins human serum albumin (HSA) and human immunoglobulin G (HIgG). A three-stage, countercurrent ultrafiltration system was able to generate continuous HSA and HIgG streams with high purity and recovery. Pulsed sample injection ultrafiltration experiments showed that selectivity could be significantly affected by protein-protein interactions. The molar ratio of proteins in the feed was affected by the sieving coefficients of the proteins, particularly that of the preferentially transmitted one. Simulated results obtained with effective sieving coefficient data from pulsed sample injection ultrafiltration which factored in protein-protein interactions were found to be in good agreement with experimental results. The selectivity and hydraulic permeability as a function of time in a continuous fractionation process run under optimized conditions were determined to examine the feasibility of operation for extended duration.
机译：本文研究了使用多级或级联超滤系统对模型蛋白人血清白蛋白（HSA）和人免疫球蛋白G（HIgG）进行连续分级的方法。三级逆流超滤系统能够产生具有高纯度和高回收率的连续HSA和HIgG物流。脉冲样品注射超滤实验表明，蛋白质间相互作用可能会极大地影响选择性。饲料中蛋白质的摩尔比受蛋白质筛分系数的影响，特别是优先传输的蛋白质的筛分系数。通过脉冲进样超滤获得的有效筛分系数数据得到的模拟结果与蛋白质相互作用被发现与实验结果非常吻合。确定了在优化条件下运行的连续分馏过程中选择性和水力渗透率随时间的变化，以检验延长操作时间的可行性。

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