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首页> 外文期刊>Separation and Purification Technology >Preparative separation and purification of alkaloids from Rhizoma coptidis by high-speed counter-current chromatography
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Preparative separation and purification of alkaloids from Rhizoma coptidis by high-speed counter-current chromatography

机译：高速逆流色谱法从黄连中生物碱的制备及分离纯化

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摘要

A fast and simple solvent system screening method was established based on average polarity, which was much easier to find a suitable solvent system for high-speed counter-current chromatography (HSCCC) separation. Six alkaloids were separated and purified simultaneously with n-hexane-ethyl acetate-methanol-water (2:5:2:5) on HSCCC in one step. As a result, 4.7 mg palmatine, 7.1 mg berberine, 0.8 mg worenine, 1.5 mg epiberberine, 1.9 mg coptisine and 0.6 mg jatrorrhizine from 20 mg Rhizoma coptidis extract were obtained with the purities of 98.5%, 94.1%, 90.4%, 95.5%, 88.4% and 91.1% respectively as determined by high-performance liquid chromatography (HPLC), and they were characterized by MS and ~1HNMR.
机译：建立了一种基于平均极性的快速简便的溶剂体系筛选方法，该方法更容易找到适用于高速逆流色谱（HSCCC）分离的溶剂体系。一步分离六种生物碱，并用正己烷-乙酸乙酯-甲醇-水（2：5：2：5）在HSCCC上同时纯化。结果从20毫克黄连提取物中获得了4.7毫克棕榈碱，7.1毫克小ber碱，0.8毫克花氨酸，1.5毫克小epi碱，1.9毫克黄连碱和0.6毫克麻黄碱，纯度为98.5％，94.1％，90.4％，95.5％通过高效液相色谱法（HPLC）测定，分别为88.4％和91.1％，并通过MS和〜1HNMR对其进行了表征。

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