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Guidelines for solvent selection for carrier mediated extraction of proteins

机译：载体介导的蛋白质提取的溶剂选择指南

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Potentially, extraction techniques offer advantages such as higher capacity, better selectivity and integration between recovery and purification. Affinity extraction with organic solvents appears promising, provided that irreversible denaturation of the extracted biomolecules can be limited. Therefore, as a first essential step the influence of various organic solvents has been studied on the extraction of typical proteins (cytochrome c, myoglobin, human serum albumin HSA) and antibody (monoclonal Immunoglobulin G (IgG)) in liquid-liquid systems. For proteins, their conformation together with internal disulfide bonds give a picture of the predictable behaviour in contact with organic solvents. Depending on the type of protein the log P or the interfacial tension can give an indication about the overall stability. In the case of IgG existing solvents and selection methods used for small proteins are not suitable to define a good solvent for IgG free of stabilizers. However, when stabilizers and sugars are present in the buffer solution almost all the solvents tested show a good capacity to retain IgG concentration. Ionic liquids demonstrated a considerably higher capacity to retain the IgG concentration than conventional organic solvents, however, do not completely stabilize monoclonal IgG in pure solution.
机译：潜在地，提取技术具有诸如更高的容量，更好的选择性以及回收和纯化之间的整合等优点。只要可以限制所提取生物分子的不可逆变性，那么用有机溶剂进行亲和萃取似乎很有希望。因此，作为第一步的重要步骤，已研究了各种有机溶剂对液-液系统中典型蛋白质（细胞色素c，肌红蛋白，人血清白蛋白HSA）和抗体（单克隆免疫球蛋白G（IgG））的提取的影响。对于蛋白质，其构象与内部二硫键一起提供了与有机溶剂接触时可预测行为的图片。取决于蛋白质的类型，log P或界面张力可以表明总体稳定性。就IgG而言，现有的用于小蛋白的溶剂和选择方法不适合定义不含稳定剂的IgG的良好溶剂。但是，当缓冲溶液中存在稳定剂和糖时，几乎所有测试的溶剂都表现出良好的保持IgG浓度的能力。与常规有机溶剂相比，离子液体显示出更高的保留IgG浓度的能力，但是，不能在纯溶液中完全稳定单克隆IgG。

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