• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Separation and Purification Technology >An innovative simultaneous glucoamylase extraction and recovery using colloidal gas aphrons
【24h】

An innovative simultaneous glucoamylase extraction and recovery using colloidal gas aphrons

机译：使用胶体气瓶同时创新地提取和回收葡糖淀粉酶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recovery and purification of biological products generally involve complicated processes that cost even up to 70% of final product price. Because of many commercial applications of glucoamylase, its innovative purification methods are still desired. In this paper, colloidal gas aphrons, generated with cationic and anionic surfactants at their critical micelle concentration (CMC), have been used to recover and purify glucoamylase enzyme from actual biomass produced by solid state fermentation. Three diverse separation methods (mixing, integrated and combined) were examined. In these tests, actual biomass samples, composed of a mixture of various proteins and enzymes, were used. Using aphron flotation, it was found that recovery of glucoamylase in a simultaneous glucoamylase extraction and recovery mode can be enhanced by using a cationic surfactant and a flocculation agent. The experimental results revealed that this innovative procedure is the most effective method in recovery and purifying glucoamylase.
机译：生物产品的回收和纯化通常涉及复杂的过程，其成本甚至高达最终产品价格的70％。由于葡糖淀粉酶的许多商业应用，仍需要其创新的纯化方法。在本文中，由阳离子和阴离子表面活性剂在其临界胶束浓度（CMC）下产生的胶体气态阿弗隆已用于从固态发酵产生的实际生物质中回收和纯化葡糖淀粉酶。研究了三种不同的分离方法（混合，整合和合并）。在这些测试中，使用了由各种蛋白质和酶的混合物组成的实际生物质样品。使用阿芙隆浮选法，发现通过使用阳离子表面活性剂和絮凝剂，可以在同时进行的葡糖淀粉酶提取和回收模式中提高葡糖淀粉酶的回收率。实验结果表明，该创新方法是回收和纯化葡糖淀粉酶的最有效方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号