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首页> 外文期刊>Separation and Purification Technology >Supported liquid membrane extraction of ~(177)Lu(III) with DEHPA and its application for purification of ~(177)Lu-DOTA-lanreotide
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Supported liquid membrane extraction of ~(177)Lu(III) with DEHPA and its application for purification of ~(177)Lu-DOTA-lanreotide

机译：支持的DEHPA液膜萃取〜（177）Lu（III）及其在〜（177）Lu-DOTA-兰瑞肽的纯化中的应用

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Permeation of ~(177)Lu(III) through supported hexane membrane containing di-(2-ethylhexyl)phosphoric acid (DEHPA) as a carrier has been studied.The donor solution was 0.2 mol dm~(-3) ammonium acetate buffer,pH 5.0-5.5,that is optimal for peptide labeling,and the acceptor solution was 2 mol dm~(-3) HC1.A miniaturized supported liquid membrane (SLM) contactor with 10 mu l donor and acceptor channel and ultra thin,flat poly(tetrafluoroethene) laminated on polyester fleece (PTFE) was applied in the work reported herein.The donor phase was pumped continuously through the donor channel,while the acceptor was stagnant during the SLM extraction.The influences of carrier concentration and donor flow rate on extraction of ~(177)Lu(III) have been investigated.The results are discussed in the term of mass transfer coefficient,mass transfer resistance and extraction efficiency.Based on the experimental results of SLM extraction of ~(177)Lu(III),this technique has been applied for the separation of free radionuclide from the labeled peptide as a purification procedure within the production of the radiopharmaceutical.An analog of somatostatin,lanreotide,which was conjugated with chelating moiety l,4,7,10-tetraazacyclododecane-N,N'N",N'"-tetraacetic acid (DOTA) was labeled with ~(177)Lu(III).The characterization of the labeled conjugate as well as the complexation yield and the efficiency of purification by membrane extraction were carried out by HPLC analysis.
机译：研究了〜（177）Lu（III）通过以二（2-乙基己基）磷酸（DEHPA）为载体的正己烷膜的渗透作用。供体溶液为0.2 mol dm〜（-3）醋酸铵缓冲液， pH 5.0-5.5，最适合于肽标记，受体溶液为2 mol dm〜（-3）HCl。微型化的支持液膜（SLM）接触器，带有10μl的供体和受体通道以及超薄，扁平的聚本文报道的工作中使用了层压在聚四氟乙烯（PTFE）上的（四氟乙烯）。供体相连续泵送通过供体通道，而在SLM萃取过程中受体停滞。载流子浓度和供体流速对萃取的影响对〜（177）Lu（III）进行了研究。从传质系数，传质阻力和萃取效率的角度对结果进行了讨论。基于〜（177）Lu（III）的SLM萃取实验结果，该技术已被应用于分离放射性药物生产过程中的纯化方法，从标记的肽中游离出放射性核素。生长抑素，兰瑞肽的类似物与螯合部分1,4,7,10-四氮杂环十二烷-N，N'N“，N'”缀合用〜（177）Lu（III）标记-四乙酸（DOTA）。通过HPLC分析进行标记共轭物的表征，络合物的收率和膜萃取的纯化效率。

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