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Multi-enzyme immobilization in eco-friendly emulsion liquid membrane reactor-a new approach to membrane formulation

机译：环保型乳化液膜反应器中多酶固定化的新方法

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摘要

Multi-enzymes (#alpha#-glucosidase and glucose oxidase) have been immobilized in emulsion liquid membranes for using such membranes as eco-friendly enzyme emulsion liquid membrane (EELM) reactors.Role of surfactant-stabilized liquid membranes as separation-concentration devices changes here to that of promising,eco-friendly reactors where product is transported to the external phase thereby eliminating the need for demlsification for subsequent recovery of product and membrane phase material for reuse.Thus tradeoffs among stability,seelctive permeability and ease of demulsification get significnalty modified necessitating a new approach to membrane formulation.With this new aproach to membrane formulation,the present investigations have been carried out.Stability aspect has been studied measuring leakage through such membranes using a tracer technique while permeability has been measured during actual enzymatic reactions inside the emulsion globules and transportationof the product outside the membranes.Effects of agitation,surfactant concentration,internal phase volume fraction,membrane phase material and non-Newtonian conversion of membrane phase on the stability of liquid membranes,as well as product generation and permeation have been investigated.Inpresence of carrier and additional stabilizer (polystyrene 1% by wt.),emulsion liquid membranes have been found to yield the best results with a surfactant concentration of 3.5-4 wt.% at an agitation of 300 rqm and with an internal phase volume fraction of 0.5.The findings are expected to be very useful in developing emulsion liquid membranes as efficient enzyme-encapsulating bioreactors.
机译：多种酶（＃alpha＃-葡萄糖苷酶和葡萄糖氧化酶）已被固定在乳化液膜中，用于诸如生态友好型酶乳化液膜（EELM）反应器之类的膜。表面活性剂稳定液膜在分离浓缩装置方面的作用发生了变化这里有希望的，对环境友好的反应器​​，其中产品被输送到外相，从而消除了脱胶的需要，从而可以随后回收产品和膜相材料以进行再利用。因此，在稳定性，渗透性和易破乳性之间的权衡取舍得到了重大改变。通过这种新的膜配方方法，进行了本研究。研究了使用示踪技术测量通过此类膜泄漏的稳定性方面，同时在乳液内部实际的酶促反应过程中测量了渗透性。小球和产品的运输研究了搅拌，表面活性剂浓度，内相体积分数，膜相材料和膜相的非牛顿转化率对液膜稳定性以及产物产生和渗透的影响。载体的存在和附加已经发现，在300 rqm的搅拌下，表面活性剂的浓度为3.5-4 wt。％，内相体积分数为0.5。作为有效的酶包封生物反应器，预期在开发乳状液膜方面非常有用。

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