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Evaluation of endospore purification methods applied to Bacillus cereus

机译：蜡样芽孢杆菌内生孢子纯化方法的评价

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Research on spore inactivation techniques requires highly pure spore suspensions,but the efficacy of commonly used purification methods has not been systematically compared.By providing this comparison,the present study will help researchers improve the consistency and validity of their experiments.The current study evaluated alternative purification methodologies applied to Bacillus cereus endospores on the basis of criteria of purity,yield,time investment and effect on integrity of spores.The ASTM E2111-00,ethanol,heat shock,daily water washes and lysozyme treatment methods were applied to B.cereus cultures incubated for 3 and 10 days.Purities achieved ranged from 67 to 99% spores as a proportion of total colony forming units in the purified suspension,whereas yields ranged from less than 1 to 39% spores in proportion to total colony forming units of B.cereus culture.TEM images showed that only lysozyme treatment substantially altered spore morphology.Ethanol treatment is optimum for 3-day B.cereus cultures,whereas the ASTM method is most favorable for 10-day-old cultures.Lysozyme treatment is the least attractive purification method because of its large time investment and damage to spores.
机译：孢子灭活技术的研究需要高度纯净的孢子悬浮液，但尚未对常用纯化方法的功效进行系统地比较。通过提供这种比较，本研究将有助于研究人员提高实验的一致性和有效性。根据纯度，产量，时间投资和对孢子完整性的影响标准，对蜡状芽孢杆菌内生孢子进行纯化。ASTME2111-00，乙醇，热激，每日水洗和溶菌酶处理方法培养物培养3天和10天。纯化后的悬浮液中，获得的纯度为总菌落形成单位的67-99％，而与B菌落形成单位的总产量相比，孢子的产生量不到1-39％蜡状菌培养.TEM图像显示，只有溶菌酶处理才能实质性改变孢子形态。 3天的蜡状芽孢杆菌培养物，而ASTM方法最适合10天龄的培养物。溶菌酶处理是最不吸引人的纯化方法，因为它的大量投资和对孢子的破坏。

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