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Novel separation of bioactive catechin derivatives from complex plant mixtures by anion-exchange chromatography

机译：阴离子交换色谱法从复杂植物混合物中新型分离生物活性儿茶素衍生物

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摘要

The paper presents the use of anion-exchange chromatography to separate bioactive cysteine containing catechin conjugates from underivatised polyphenols and oligomeric material. The conjugates were excluded from the anion-exchange resin using a low percentage of organic solvent, enough to eliminate the weak hydrophobic interactions with the matrix. The rest of the uncharged polyphenols were unexpectedly retained by the matrix and eluted by the addition of salt. Cation-π interactions between the quaternary amino group on the resin and the electron-rich faces of the flavanol's aromatic rings might be responsible for this unexpected behaviour. Because immobilized anions retain uncharged polyphenolic material, anion-exchange chromatography may be a convenient approach to separate polyphenols from other bioactive compounds in complex mixtures of plant origin.
机译：本文介绍了阴离子交换色谱法从未衍生的多酚和低聚物质中分离出具有生物活性的半胱氨酸儿茶素结合物的方法。使用低百分比的有机溶剂将共轭物从阴离子交换树脂中排除，足以消除与基质之间的弱疏水相互作用。其余的不带电荷的多酚被基质意外地保留，并通过添加盐洗脱。树脂上的季氨基与黄烷醇芳环的富电子面之间的阳离子-π相互作用可能是这种意外行为的原因。由于固定的阴离子保留了不带电荷的多酚类物质，因此阴离子交换色谱法可能是从植物来源的复杂混合物中将多酚与其他生物活性化合物分离的便捷方法。

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