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首页> 外文期刊>Separation and Purification Technology >Purification of quercetin in Anoectochilu roxburghii (wall) Lindl using UMAE by high-speed counter-current chromatography and subsequent structure identification
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Purification of quercetin in Anoectochilu roxburghii (wall) Lindl using UMAE by high-speed counter-current chromatography and subsequent structure identification

机译：UMAE高速逆流色谱法纯化罗克氏不动杆菌（壁）Lindl中的槲皮素

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摘要

Quercetin is a major phenolic compound in the Chinese medicinal herb. It has been proved to have many important pharmacological active compounds. To the best of our knowledge, this is the first paper to report that quertin exists in Anoectochilu roxburghii (wall) Lindl. The high-speed counter-current chromatography (HSCCC) was successfully applied to purify quercetin from the extracts of A roxburghii (wall) Lindl after ultrasound-microwave assisted extraction. The two-phase solvent system used in HSCCC was n-hexane-ethyl acetate-methanol-water (4:6:3:3, v/v/v/v). The purity of the obtained quercetin was over 96.8%, i.e. 30.8 mg quercetin was obtained from 6.02 g of the crude extract. Its chemical structure was identified by using UV spectra, FT-IR, electrospray ionization mass spectrometry (ESI-MS) and ~1H NMR. The results showed that ultrasound-microwave assisted extraction was a promising method for extracting quercetin, and HSCCC was a feasible method to be used in the purification of quercetin from the herb. This separation method was more effective than some conventional techniques.
机译：槲皮素是中草药中的主要酚类化合物。已证明具有许多重要的药理活性化合物。据我们所知，这是第一篇报道槲皮素存在于Anoectochilu roxburghii（wall）Lindl中的论文。高速逆流色谱法（HSCCC）成功地应用于超声波微波辅助提取后的A roxburghii（壁）Lindl提取物中的槲皮素。 HSCCC中使用的两相溶剂系统是正己烷-乙酸乙酯-甲醇-水（4：6：3：3，v / v / v / v）。所得槲皮素的纯度超过96.8％，即从6.02g的粗提物中得到30.8mg的槲皮素。通过使用紫外光谱，傅立叶变换红外光谱，电喷雾电离质谱（ESI-MS）和〜1H NMR鉴定了其化学结构。结果表明，超声波辅助提取是提取槲皮素的一种有前途的方法，而HSCCC是从草药中纯化槲皮素的可行方法。这种分离方法比某些常规技术更有效。

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