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首页> 外文期刊>Separation and Purification Technology >Concentration and pre-purification with ultrafiltration of a R-phycoerythrin solution extracted from macro-algae Grateloupia turuturu: Process definition and up-scaling
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Concentration and pre-purification with ultrafiltration of a R-phycoerythrin solution extracted from macro-algae Grateloupia turuturu: Process definition and up-scaling

机译：从大型藻类Grateloupia turuturu提取的R-藻红蛋白溶液的超滤浓缩和预纯化：过程定义和放大

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摘要

Grateloupia turuturu, an invasive red macro-alga, represents an unexploited important biomass. A potential use of this seaweed is the production of valuable molecules, such as R-phycoerythrin. Nevertheless, after R-phycoerythrin extraction, protein extract has to be concentrated and pre-purified, which is generally expensive and requires many stages. The present study deals with the feasibility to implement a single stage of ultrafiltration for this purpose. A membrane screening was first realized at a lab-scale (Amicon stirred cell) to determine a suitable membrane molecular weight cut-off and a material allowing an optimal concentration and pre-purification of R-phycoerythrin contained in a hydrosoluble extract The best adapted membrane turned out to be a polyethersulfone 30 kDa one. An up-scaling methodology was then realized on a PCI Microlab40 pilot plant equipped with an industrial type polyethersulfone 25-30 kDa membrane. The results show that R-phycoerythrin was concentrated without denaturation and accumulation of undesired molecules. Ultrafiltration up to a volume reduction factor of 5 was effective for R-phycoerythrin concentration and pre-purification: 100% of R-phycoerythrin was recovered, 32.9% of other proteins and 64.6% of sugars passed through membrane. Based on these results, a techno-economic study was investigated on an ultrafiltration unit at an industrial scale.
机译：Grateloupia turuturu是一种入侵性红色巨藻，代表未开发的重要生物质。这种海藻的潜在用途是产生有价值的分子，例如R-藻红蛋白。然而，在R-藻红蛋白提取后，蛋白质提取物必须进行浓缩和预纯化，这通常很昂贵并且需要很多步骤。本研究探讨了为此目的实施单级超滤的可行性。首先在实验室规模（Amicon搅拌池）上进行膜筛选，以确定合适的膜分子量截断值，以及可以对水溶性提取物中所含R-藻红蛋白进行最佳浓度和预纯化的材料。原来是30 kDa的聚醚砜。然后在配备有工业型聚醚砜25-30 kDa膜的PCI Microlab40中试装置上实现了放大方法。结果表明，R-藻红蛋白被浓缩而没有不期望的分子的变性和积累。超滤至体积减少因子5时，对R-藻红蛋白的浓度和预纯化有效：回收了100％的R-藻红蛋白，32.9％的其他蛋白质和64.6％的糖通过膜。基于这些结果，对工业规模的超滤装置进行了技术经济研究。

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