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首页> 外文期刊>Separation and Purification Technology >Purification of protease from pre-treated tuna spleen extract by ultrafiltration: An altered operational mode involving critical flux condition and diafiltration
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Purification of protease from pre-treated tuna spleen extract by ultrafiltration: An altered operational mode involving critical flux condition and diafiltration

机译：通过超滤从预处理的金枪鱼脾提取物中纯化蛋白酶：一种改变的操作模式，涉及临界通量条件和渗滤

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摘要

Diafiltrations by altered operational modes were applied to improve protease purity during ultrafiltration of pre-treated tuna spleen extract. The performance of mode 1 (pre-diafiltration followed by post-concentration) and mode 2 (pre-concentration followed by post-diafiltration) was compared. A critical flux operation was involved in mode 1. Severe fouling was avoided in mode 1 while 12-fold purification of protease was achieved after an initial diafiltration volume of 3 and a final volume concentration factor of 2 were obtained with a total operational time of about 4 h. The conventional operation, that is mode 2, provided 2-fold purification of protease after having obtained an initial volume concentration factor of 2 and a final diafiltration volume of 3. Fouling was much more severe in mode 2 than in mode 1. Consequently, low flux caused by fouling led to a long operational time of about 9 h in mode 2. The internal membrane fouling was due to the adsorption of molecules onto the membrane pore wall and pore blocking by protein or other solutes in both mode 1 and mode 2. The difference in resistance between mode 1 and mode 2 was mainly due to concentration polarization and external membrane fouling. In this investigation it was established that operation of diafiltration with critical flux concept could provide a more efficient purifying process than the conventional diafiltration operation. The reduction of fouling not only improved process efficiency and protease purity, it also possibly further reduced the cost of membrane cleaning.
机译：通过改变操作模式进行的渗滤可在预处理金枪鱼脾提取物的超滤过程中提高蛋白酶的纯度。比较了模式1（渗滤前，后浓缩）和模式2（浓缩前，后渗滤）的性能。模式1涉及临界通量操作。在模式1中避免了严重的结垢，而在最初的渗滤体积为3和最终体积浓缩因子为2且总操作时间约为10分钟后，实现了12倍的蛋白酶纯化。 4小时常规操作，即模式2，在获得初始体积浓度因子2和最终渗滤体积3后，提供了2倍的蛋白酶纯化。模式2中的结垢比模式1中严重得多。在模式2下，由结垢引起的通量导致较长的运行时间，约为9 h。内部膜结垢是由于在模式1和模式2下分子吸附到膜孔壁上以及蛋白质或其他溶质阻塞了孔。模式1和模式2之间的电阻差异主要是由于浓差极化和外部膜污染。在这项研究中，确定了具有临界通量概念的渗滤操作可以提供比常规渗滤操作更有效的纯化过程。减少结垢不仅提高了工艺效率和蛋白酶纯度，还可能进一步降低了膜清洗的成本。

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