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首页> 外文期刊>Separation and Purification Technology >A novel technique for the separation and concentration of penicillin G from fermentation broth: Aqueous two-phase flotation
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A novel technique for the separation and concentration of penicillin G from fermentation broth: Aqueous two-phase flotation

机译：从发酵液中分离和浓缩青霉素G的新技术：水两相浮选

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摘要

Separation by solvent sublation has been used for the first time in an aqueous two-phase system, and a new concept, aqueous two-phase flotation (ATPF), is proposed. The new technique was used to separate and concentrate penicillin G from a fermentation broth. In an ATPF system of polyethylene glycol (PEG)/ammonium sulfate, the effects of varying solution pH, concentration of ammonium sulfate in aqueous solution, nitrogen flow rate, flotation time and initial volume of the PEG phase were investigated in detail, and the optimal conditions for ATPF were obtained. Under these optimal conditions, the separation efficiency of ATPF was more than 95%. Compared with liquid-liquid extraction, solvent sublation and aqueous two-phase extraction, ATPF is shown to be an effective way of separating and concentrating penicillin G from an aqueous phase, and also gives a reduced consumption of organic solvents.
机译：在水两相系统中，溶剂浮选分离已首次使用，提出了一种新概念，即水两相浮选（ATPF）。新技术用于从发酵液中分离和浓缩青霉素G。在聚乙二醇（PEG）/硫酸铵的ATPF系统中，详细研究了溶液pH值，水溶液中硫酸铵的浓度，氮气流速，浮选时间和PEG相初始体积的影响，并研究了最佳获得了ATPF的条件。在这些最佳条件下，ATPF的分离效率超过95％。与液-液萃取，溶剂升华和水两相萃取相比，ATPF被证明是从水相中分离和浓缩青霉素G的有效方法，并且减少了有机溶剂的消耗。

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