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首页> 外文期刊>Separation and Purification Technology >Comparative study on different strategies involved for xylitol purification from culture media fermented by Candida tropicalis
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Comparative study on different strategies involved for xylitol purification from culture media fermented by Candida tropicalis

机译：从热带念珠菌发酵培养基中纯化木糖醇的不同策略的比较研究

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摘要

Xylitol, a sugar substitute, is a high value product for pharmaceutical and food industries and its purification being of commercial importance. In the present study, the purification of xylitol obtained through Candida tropicalis by fermentation using synthetic xylose and corn cob hemicellulosic hydrolysate as substrates were studied for liquid-liquid extraction (21.72 g/l xylitol extracted in 1:5 (v/v) of ethyl acetate) and precipitation (67.44% xylitol recovery along with certain impurities). By this method xylitol recovery is difficult and expensive for large scale processes. Therefore, activated charcoal treatment followed by vaccum concentration and crystallization method for xylitol extraction was evaluated. The optimized conditions obtained for activated charcoal treatment followed by vaccum concentration and crystallization method were 15.0g/l of charcoal concentration at 30°C for 1 h with 10 times super saturation of initial concentration and crystallization temperature of -20 °C for initiation and then at 8°C yielding 43.97%. After 4 cycles of crystallization, 76.20% and 68.06% xylitol crystallization yield was obtained in 50 ml and 5.01 of the synthetic xylose fermentation broth by adapted strain of C. tropicalis respectively. The effect of solvents on the crystalline structure of xylitol showed prismatic structure in the presence of ethanol and orthorhombic needles in the presence of tetrahydrofuran. The purity of the xylitol was characterized using ~(13)C and ~1H nuclear magnetic resonance, mass spectroscopy, and optical rotation, confirming 98.99% purity in a pure crystallized form.
机译：木糖醇是糖的替代品，是制药和食品工业的高价值产品，其提纯具有商业重要性。在本研究中，以合成木糖和玉米芯半纤维素水解物为底物，通过发酵纯化热带假丝酵母得到的木糖醇，进行了液-液萃取（21.72 g / l木糖醇，以1：5（v / v）的乙基乙醇萃取）醋酸盐）和沉淀（67.44％的木糖醇回收率以及某些杂质）。通过这种方法，木糖醇的回收对于大规模过程而言是困难且昂贵的。因此，评估了活性炭处理，真空浓缩和木糖醇提取的结晶方法。活性炭处理后再进行真空浓缩和结晶方法得到的最佳条件是：在30°C下1h的木炭浓度为15.0g / l，初始浓度为原始饱和度的10倍，起始温度为-20°C，然后再结晶。在8℃下产率为43.97％。经过4个结晶周期，通过适应的热带假丝酵母菌株分别在50ml和5.01的合成木糖发酵液中获得了76.20％和68.06％的木糖醇结晶产率。溶剂对木糖醇晶体结构的影响在乙醇存在下呈棱柱状结构，在四氢呋喃存在下呈正交晶状针状结构。使用〜（13）C和〜1H核磁共振，质谱和旋光度表征木糖醇的纯度，从而以纯结晶形式确认纯度为98.99％。

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