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首页> 外文期刊>Chembiochem: A European journal of chemical biology >Investigation of a 6-MSA Synthase Gene Cluster in Aspergillus aculeatus Reveals 6-MSA-derived Aculinic Acid, Aculins A-B and Epi-Aculin A
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Investigation of a 6-MSA Synthase Gene Cluster in Aspergillus aculeatus Reveals 6-MSA-derived Aculinic Acid, Aculins A-B and Epi-Aculin A

机译：刺曲霉中6-MSA合酶基因簇的研究揭示了6-MSA衍生的乙酸，Aculins A-B和Epi-Aculin A

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摘要

Aspergillus aculeatus, a filamentous fungus belonging to the Aspergillus clade Nigri, is an industrial workhorse in enzyme production. Recently we reported a number of secondary metabolites from this fungus; however, its genetic potential for the production of secondary metabolites is vast. In this study we identified a 6-methylsalicylic acid (6-MSA) synthase from A. aculeatus, and verified its functionality by episomal expression in A. aculeatus and heterologous expression in A. nidulans. Feeding studies with fully C-13-labeled 6-MSA revealed that 6-MSA is incorporated into aculinic acid, which further incorporates into three compounds that we name aculins A and B, and epi-aculin A, described here for the first time. Based on NMR data and bioinformatic studies we propose the structures of the compounds as well as a biosynthetic pathway leading to formation of aculins from 6-MSA.
机译：刺曲霉（Aspergillus aculeatus）是属于曲霉属Nigri的一种丝状真菌，是酶生产中的工业动力。最近，我们报道了该真菌的许多次生代谢产物。然而，其产生次级代谢产物的遗传潜力是巨大的。在这项研究中，我们从A. aculeatus中鉴定了6-甲基水杨酸（6-MSA）合酶，并通过A. aculeatus中的游离表达和ni.lanus中的异源表达验证了其功能。用完全C-13标记的6-MSA进行的饲喂研究表明，6-MSA被掺入了阿奇林酸中，后者进一步掺入了我们首次命名为aculins A和B的三种化合物以及表阿霉素A。根据NMR数据和生物信息学研究，我们提出了化合物的结构以及导致6-MSA形成aculins的生物合成途径。

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