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首页> 外文期刊>Sexually Transmitted Infections >Contamination of environmental surfaces by genital human papillomaviruses.
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Contamination of environmental surfaces by genital human papillomaviruses.

机译：生殖器人乳头瘤病毒对环境表面的污染。

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摘要

OBJECTIVE: To investigate contamination of environmental surfaces with human papillomaviruses (HPV) DNA in two genitourinary medicine (GUM) clinics and in an on-site staff leisure and fitness centre. METHODS: Samples were collected from the treatment rooms and patients' toilets of two GUM clinics situated at two hospital sites and from the toilets of the staff leisure and fitness centre on one of the sites. Samples were tested for the presence of HPV DNA by nested polymerase chain reaction (PCR), and HPV amplicons were typed by reverse line hybridisation using HPV type specific oligonucleotide probes complementary to 35 HPV types. All samples were also tested for beta globin DNA by PCR in order to assess their quality. RESULTS: HPV DNA was found to be present at more than 50% of the sites sampled in one of the GUM clinics, but was absent in the second, and also from the staff leisure and fitness centre. All HPV DNA detected was found to be cell associated. The most commonly found HPV types were 6, 11, and 16, respectively. HPV infected cells were found to be localised mainly to surfaces used predominantly by medical staff. CONCLUSIONS: This study has identified contamination of the environment of a GUM clinic. Possible sources for the contamination of the clinic toilets were from genital sites via hands to the environment. Within the treatment rooms the most likely route of HPV DNA contamination of the environment was via the doctor's gloved hands.
机译：目的：在两家泌尿生殖医学（GUM）诊所以及员工休闲和健身中心，调查人乳头瘤病毒（HPV）DNA对环境表面的污染。方法：从位于两个医院站点的两个口香糖诊所的治疗室和患者洗手间以及其中一个站点的员工休闲和健身中心的洗手间中收集样本。通过巢式聚合酶链反应（PCR）测试样品中HPV DNA的存在，并使用与35种HPV类型互补的HPV类型特异性寡核苷酸探针通过反向杂交对HPV扩增子进行分型。还通过PCR测试了所有样品的β珠蛋白DNA，以评估其质量。结果：在一家口香糖诊所中，发现HPV DNA存在于超过50％的采样点中，但在第二家中以及工作人员休闲和健身中心中都没有。发现所有检测到的HPV DNA与细胞相关。最常见的HPV类型分别为6、11和16。发现被HPV感染的细胞主要定位在主要由医务人员使用的表面上。结论：本研究确定了口香糖诊所环境的污染。可能从诊所的生殖器部位通过手到环境来污染诊所的厕所。在治疗室中，HPV DNA污染环境的最可能途径是通过医生戴着手套的手。

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