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首页> 外文期刊>Biomaterials >Natural and artificial substrates for retinal pigment epithelial monolayer transplantation.
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Natural and artificial substrates for retinal pigment epithelial monolayer transplantation.

机译：视网膜色素上皮单层移植的天然和人工基质。

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摘要

The aim of this study was to culture retinal pigment epithelial (RPE) cells on natural and synthetic substrates for future use in RPE monolayer transplantation in the eye. The extracellular capsules surrounding the human lens and a hydrogel biomaterial were used as substrates for monolayer culture. All materials were seeded with either pig or human retinal pigment epithelial cells and were maintained in tissue culture conditions. Upon confluency, the cell density was calculated and cell viability determined. All monolayers were stained with phalloidin-rhodamine for F-actin and antibodies to tight junction-associated protein, ZO1. The final cell density of human RPE monolayers on the hydrogel and lens capsule was 3,200 +/- 187 and 3,350 +/- 120 cells/mm2 respectively. Pig RPE cells had a final cell density of 3,740 +/- 20 5cells/mm2 on the lens capsule and 3,025+ cells/mm2 on the hydrogel. F-actin staining revealed a circumferential ring of actin filaments in all the cells grown on substrates. ZO1 immunohistochemisty demonstrated staining along the lateral cell borders of all cell types. The successful culture of RPE cells on these substrates may have the potential for transplanting cell monolayers in the eye to improve outcomes for degenerative diseases in the retina.
机译：这项研究的目的是在天然和合成基质上培养视网膜色素上皮（RPE）细胞，以备将来在眼中进行RPE单层移植时使用。围绕人晶状体的细胞外胶囊和水凝胶生物材料被用作单层培养的底物。所有材料均接种猪或人视网膜色素上皮细胞，并保持在组织培养条件下。融合后，计算细胞密度并确定细胞活力。所有单层均用鬼笔环肽-若丹明染色，以检测F-肌动蛋白和与紧密连接相关的蛋白质ZO1的抗体。在水凝胶和晶状体囊上，人RPE单层的最终细胞密度分别为3,200 +/- 187和3,350 +/- 120细胞/ mm2。猪RPE细胞的最终细胞密度在晶状体囊上为3,740 +/- 20 5细胞/ mm2，在水凝胶上为3,025+细胞/ mm2。 F-肌动蛋白染色显示在底物上生长的所有细胞中肌动蛋白丝的圆周环。 ZO1免疫组化显示沿所有细胞类型的侧向细胞边界染色。在这些基质上成功培养RPE细胞可能具有将细胞单层移植到眼中的潜力，从而可以改善视网膜退行性疾病的预后。

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