...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials >XPS and surface-MALDI-MS characterisation of worn HEMA-based contact lenses.
【24h】

XPS and surface-MALDI-MS characterisation of worn HEMA-based contact lenses.

机译：磨损的HEMA隐形眼镜的XPS和MALDI-MS表面表征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

XPS and MALDI-MS were used to analyse initial adsorption events in the fouling of HEMA-based contact lenses. All of the lenses tested accumulated tear film deposits within 10 min of wear. XPS indicated the presence of mainly proteinaceous deposits, with indications of some contributions by mucins or lipids on some lenses and the nature of the deposit being influenced by the lens chemistry. MALDI-MS detected the presence of surface-adsorbed species with molecular weights < 15 kDa. While lysozyme could be identified by comparison of MALDI-MS signals with known protein mass and assignments are suggested for some other signals, several other species, with MWs less than that of lysozyme, could not be identified as no ocular proteins with corresponding MWs had been reported in previous biochemical tear film analyses. These species, and others, were also detected in MALDI-MS analysis of reflex tear film, suggesting that the adsorbed unidentified species were not simply products of surface-induced dissociation of adsorbing higher-MW proteins. This short-term wear study detected rapid interface conversion and demonstrated the utility and surface sensitivity of XPS and MALDI-MS in characterising contact lens deposits at the initial stages when sub-monolayer adsorbed amounts are present on lenses.
机译：XPS和MALDI-MS用于分析基于HEMA的隐形眼镜结垢中的初始吸附事件。所有经过测试的镜片在佩戴后10分钟内就会积累泪膜沉积物。 XPS指出主要是蛋白质沉积物的存在，并表明粘蛋白或脂质在某些晶状体上的某些贡献，并且该沉积物的性质受晶状体化学性质的影响。 MALDI-MS检测到分子量小于15 kDa的表面吸附物质的存在。虽然可以通过将MALDI-MS信号与已知蛋白质质量进行比较来鉴定溶菌酶，并建议对其他一些信号进行分配，但无法鉴定出分子量小于溶菌酶的其他几种菌种，因为尚未鉴定出具有相应分子量的眼用蛋白。在以前的生化泪膜分析中已有报道。这些物种和其他物种也在反射泪膜的MALDI-MS分析中被检测到，表明所吸附的未识别物种并非仅仅是表面诱导的高分子量蛋白质吸附解离的产物。这项短期磨损研究检测到了快速的界面转换，并证明了XPS和MALDI-MS在表征隐形眼镜沉积物的初始阶段（当镜片上存在亚单层吸附量时）具有实用性和表面敏感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号