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首页> 外文期刊>Biomaterials >Behavior of osteoblasts on a type I atelocollagen grafted ozone oxidized poly L-lactic acid membrane.
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Behavior of osteoblasts on a type I atelocollagen grafted ozone oxidized poly L-lactic acid membrane.

机译：成骨细胞在I型端胶原胶原接枝的臭氧氧化聚L-乳酸膜上的行为。

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With oxidizing poly-L-lactic acid (PLLA) surface by ozone, peroxide groups are easily generated on the surface. Those peroxides are broken down by redox-coupling reaction, and provide active species that initiate grafting by reaction with the collagen molecules. The surface density of generated peroxide on a PLLA surface was determined by an iodide method. The maximum concentration of peroxide was about 2.87 x 10(-8) mol/cm2 when ozone oxidation was performed at 60 V for 60 min. After the surface oxidation, type I atelocollagen was grafted onto PLLA surface. All physical measurements on the collagen-grafted surface indicated that the PLLA surface was effectively grafted with type I atelocollagen. Behavior of rat calvaria osteoblasts on type I atelocollagen grafted PLLA (PLLA + COL) surface was observed. Initial attachment of osteoblasts on the surface was significantly enhanced, and it is assumed that the atelocollagen matrix supported the initial attachment and growth of cells. Collagenous protein synthesis of osteoblasts was maintained at relatively low level in the early stage of proliferation due to the primarily existing grafted type I atelocollagen, and then increased in 7 days as the osteoblast differentiated. After 7 days, collagenous protein synthesis in osteoblasts was activated. Alkaline phosphatase (ALPase) activity and mineralization by osteoblasts were promoted on PLLA + COL surface. In comparison with PLLA + COL, non-treated PLLA and tissue culture plate (TCPS) did not show any feature expressed in osteoblasts' maturation up to 9 days in this experiment. The grafted type I atelocollagen provided a favorable matrix for cell migration in relation with collagenase expression. Ozone oxidation might be a favorable method for surface modification of PLLA membranes by collagen grafting, and cell behavior could be modulated by the grafted collagen.
机译：通过臭氧氧化聚-L-乳酸（PLLA）表面，容易在表面上产生过氧化物基团。这些过氧化物通过氧化还原偶联反应被分解，并提供通过与胶原分子反应而引发接枝的活性物质。通过碘化物法测定PLLA表面上产生的过氧化物的表面密度。当在60 V下进行60分钟臭氧氧化时，过氧化物的最大浓度约为2.87 x 10（-8）mol / cm2。表面氧化后，将I型胶原蛋白移植到PLLA表面。在胶原移植表面上的所有物理测量表明，PLLA表面已有效地被I型胶原蛋白移植。观察到大鼠颅骨成骨细胞在I型胶原胶原移植的PLLA（PLLA + COL）表面上的行为。成骨细胞在表面上的初始附着显着增强，并且假定端粒胶原蛋白基质支持细胞的初始附着和生长。成骨细胞的胶原蛋白合成在增殖的早期由于最初存在的嫁接的I型端粒胶原蛋白而维持在相对较低的水平，然后随着成骨细胞的分化在7天内增加。 7天后，成骨细胞中的胶原蛋白合成被激活。碱性磷酸酶（ALPase）的活性和成骨细胞的矿化作用在PLLA + COL表面得到促进。与PLLA + COL相比，未经处理的PLLA和组织培养板（TCPS）在此实验中直到9天都没有表现出成骨细胞成熟所表达的任何特征。移植的I型胶原蛋白为胶原蛋白的表达提供了细胞迁移的良好基质。臭氧氧化可能是通过胶原接枝对PLLA膜进行表面改性的一种有利方法，并且可以通过嫁接的胶原来调节细胞行为。

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