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首页> 外文期刊>Chemia Analityczna >Flow-injection Spectrophotometric Determination of Tiopronin Based on Coupled Redox-Complexation Reaction
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Flow-injection Spectrophotometric Determination of Tiopronin Based on Coupled Redox-Complexation Reaction

机译：偶合氧化还原络合反应流动注射分光光度法测定硫普罗宁

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摘要

A flow-injection spectrophotometric procedure for determination of N-(2-mercapto-propionyl)-glycine (MPG) tropronin has been proposed. Determination was based on the coupled redox-complexation reaction between MPG, Fe(III) and 2,4,6-trypyridyl-S-triaz-ine (TPTZ). Firstly, MPG reduced Fe(III) to Fe(II), which was rapidly converted to the highly stable, deep-blue coloured Fe(TPTZ)~(2+). This coloured complex was monitored at 593 nm. Optimal conditions for determination of MPG were obtained applying the univariate method. A linear calibration curve was established in MPG concentration range 6.0 × 10~(-6)-2.0 × 10~(-4) mol L~(-1) with regression equation y = 4675 × - 0.0195 (R~2 = 0.9999) (n = 9) and detection limit of 4.0 × 10~(-6). Analytical frequency was 60 h~(-1). The proposed method was simple, rapid, sensitive and reproducible (RSD 0.5%, n = 10). It can be applied to determination of up to nanomolar levels of MPG in pharmaceutical preparations. Usual excipients used as additives in pharmaceuticals did not interfere with determination.
机译：提出了一种流动注射分光光度法测定N-（2-巯基-丙酰基）-甘氨酸（MPG）肌钙蛋白的方法。测定基于MPG，Fe（III）和2,4,6-吡啶基-S-三嗪-胺（TPTZ）之间的偶联氧化还原络合反应。首先，MPG将Fe（III）还原为Fe（II），然后迅速转变为高度稳定的深蓝色Fe（TPTZ）〜（2+）。在593nm处监测该有色复合物。使用单变量方法获得测定MPG的最佳条件。建立MPG浓度范围6.0×10〜（-6）-2.0×10〜（-4）mol L〜（-1）的线性校准曲线，回归方程y = 4675×-0.0195（R〜2 = 0.9999） （n = 9），检出限为4.0×10〜（-6）。分析频率为60 h〜（-1）。所提出的方法简单，快速，灵敏且可重现（RSD 0.5％，n = 10）。它可用于测定药物制剂中高达纳摩尔水平的MPG。在药物中用作添加剂的常用赋形剂不会干扰测定。

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