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首页> 外文期刊>Small Fruits Review >In Vitro Culture of Lowbush Blueberry (Vaccinium angustifolium Ait.)
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In Vitro Culture of Lowbush Blueberry (Vaccinium angustifolium Ait.)

机译：Lowbush蓝莓的体外培养（Vaccinium angustifolium Ait。）

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摘要

Cultures of three lowhush blueberry (Vaccinium angustifolium Ait.) clones collected from natural stands in Newfoundland were established in vitro on a modified cranberry (V. macrocarpon Ait.) tissue culture medium containing zeatin (5 mu M) or N~6-[2-isopentenyl]adenine (2iP) (10 mu M). Shoot proliferation with respect to shoot number per explant differed among clones at various concentrations of zeatin over two culture periods. Best total shoot proliferation was obtained when basal nodal segments were cultured in the medium supplemented with 2-4 mu M zeatin. In another experiment, nodal explants were more productive than shoot tips. Shoots growing for more than 12 weeks on media that contained more than 4 uM zealin occasionally produced adventitious shoot masses, which appeared to arise from dense calli growing at the base of the shoots in the medium. The lower concentration of sucrose and lower irradiance improved shoot proliferation with respect to vigor compared to the control treatments (30 gL~(-1) and 30 mu mol m~(-2)s~(-1), respectively). In all experiments with subculture, there was an increase in shoot multiplication rale lor all clones. A 50-100-fold multiplication rate was obtained every 3 months with the clones.
机译：在含有玉米蛋白（5μM）或N〜6- [2]的改良蔓越莓（V. macrocarpon Ait。）组织培养基上体外建立了从纽芬兰天然林中收集的三个低矮蓝莓（Vaccinium angustifolium Ait。）克隆的培养。 -异戊烯基]腺嘌呤（2iP）（10μM）。在两个培养期中，不同玉米蛋白浓度的克隆中，相对于每个外植体的芽数而言，芽增殖不同。当在添加2-4μM玉米素的培养基中培养基础节段时，可获得最佳的总芽增殖。在另一个实验中，结点外植体比发梢高产。在含有超过4 uM zealin的培养基上生长超过12周的芽偶尔会产生不定芽，这似乎是由于培养基中芽基部生长的致密愈伤组织引起的。与对照相比（分别为30 gL〜（-1）和30μmol m〜（-2）s〜（-1）），较低的蔗糖浓度和较低的辐照度提高了芽的活力。在所有传代培养实验中，所有克隆的芽繁殖规则都增加了。每三个月，克隆获得50-100倍的繁殖率。

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