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首页> 外文期刊>Biomaterials >Bacterial interactions with contact lenses; effects of lens material, lens wear and microbial physiology.
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Bacterial interactions with contact lenses; effects of lens material, lens wear and microbial physiology.

机译：与隐形眼镜的细菌相互作用;镜片材料，镜片磨损和微生物生理学的影响。

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Contact lens wear is a successful form of vision correction. However, adverse responses can occur during wear. Many of these adverse responses are produced as a consequence of bacterial colonization of the lens. The present study demonstrated that during asymptomatic contact lens wear lenses are colonized by low levels of bacteria with gram-positive bacteria, such as coagulase negative staphylococci, predominating. Gram-negative bacteria are frequently the causative agents of adverse responses during contact lens wear. Measuring the adhesion of different strains and/or species of bacteria to different contact lens materials demonstrated considerable differences. In particular. Pseudormonas aeruginosa strains Paerl and 6294 and Aeromonas hydrophilia strain Ahyd003 adhered in larger numbers to the highly oxygen permeable contact lenses Balafilcon A compared to hydrogel lenses manufactured from either Etafilcon A or HEMA. Furthermore, after Balafilcon A lenses had been worn for 6 h during the day bacteria were able to adhere in greater numbers to the worn lenses compared to the unworn lenses with increases in adhesion ranging from 243% to 1393%. However, wearing Etafilcon A lenses usually resulted in a decrease in adhesion (22-48%). Bacteria were able to grow after adhesion to lenses soaked in artificial tear fluid and formed biofilms, visualized by scanning confocal microscopy. Chemostat grown bacterial cultures were utilized to enable control of bacterial growth conditions and bacteria were shown to adhere in the greatest numbers if grown under low temperature (25 degrees C compared to 37 degrees C). The changes in growth temperature was shown. using 2D gel electrophoresis, to change the experssion of cell-surface proteins and, using ID gel electrophoresis, to change the expression of surface lipopolysaccharide of P. aeruginosa Paerl. Thus, these surface changes would have been likely to have mediated the increased adhesion to Etafilcon A contact lenses.
机译：配戴隐形眼镜是矫正视力的成功形式。但是，在穿着过程中可能发生不良反应。这些不良反应中的许多是由于晶状体细菌定植而产生的。本研究表明，在无症状的隐形眼镜佩戴过程中，以低水平的细菌定植的革兰氏阳性细菌（例如凝固酶阴性葡萄球菌）居多。革兰氏阴性菌通常是隐形眼镜佩戴过程中不良反应的诱因。测量不同菌株和/或细菌种类对不同隐形眼镜材料的粘附力显示出相当大的差异。特别是。与由Etafilcon A或HEMA制造的水凝胶镜片相比，铜绿假单胞菌Paerl和6294菌株和嗜水气单胞菌Ahyd003菌株大量附着在高透氧性隐形眼镜Balafilcon A上。此外，与未佩戴的镜片相比，在一天中佩戴Balafilcon A镜片6小时后，细菌能够更多地粘附在佩戴的镜片上，粘附力从243％增至1393％。但是，佩戴Etafilcon A镜片通常会导致粘合力下降（22-48％）。细菌粘附到浸泡在人工泪液中的晶状体上后能够生长，并形成生物膜，通过扫描共聚焦显微镜观察。利用恒化细菌生长的细菌培养物来控制细菌的生长条件，并且显示出如果在低温下（25摄氏度比37摄氏度）生长，细菌会粘附最多。显示了生长温度的变化。使用2D凝胶电泳来改变细胞表面蛋白的表达，并使用ID凝胶电泳来改变铜绿假单胞菌表面脂多糖的表达。因此，这些表面变化很可能已介导了对依他菲康A隐形眼镜的附着力增加。

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