Inhibition of goat sperm-zona binding by monoclonal antibodies to a glycoprotein family (ZP4) of porcine zona pellucida.

Gautam Kaul; Gandhi KK; Kaul G

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Inhibition of goat sperm-zona binding by monoclonal antibodies to a glycoprotein family (ZP4) of porcine zona pellucida.

机译：单克隆抗体对猪透明带糖蛋白家族（ZP4）的单克隆抗体抑制山羊精子-zona结合。

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The glycoproteins of heat-solubilized goat zona pellucida (gZP) were isolated into 3 families by HPLC on a gel filtration column. The molecular weights were 180, 95 and 75 kDa for gZP1, gZP2 and gZP3 respectively. However, under reducing conditions,4 zona pellucida fractions of 135, 95, 75 and 42 kDa were observed. The monoclonal antisera Mab-5H4 against porcine ZP4 (a 23 kDa glycoprotein) easily recognized the 180 and 42 kDa gZP fractions. The 5H4 antisera strongly inhibited homologous goat sperm-zona binding. Using immunofluorescent staining, the goat oocytes reacted positively with the 5H4 antisera. The fluorescence was uniform at 1:10 antisera dilution but tended to fade at higher dilutions. It is concluded that gZP has an antigen epitope correlative to the 5H4 antisera which is involved in sperm-zona interaction and could be a good candidate for the development of a contraceptive vaccine.

机译：通过凝胶过滤柱上的HPLC，将热增溶的山羊透明带（gZP）的糖蛋白分为3个家族。 gZP1，gZP2和gZP3的分子量分别为180、95和75 kDa。然而，在还原条件下，观察到4个透明带，分别为135、95、75和42 kDa。针对猪ZP4（23 kDa糖蛋白）的单克隆抗血清Mab-5H4容易识别180和42 kDa gZP组分。 5H4抗血清强烈抑制同源山羊精子-zona结合。使用免疫荧光染色，山羊卵母细胞与5H4抗血清反应阳性。在1:10的抗血清稀释度下，荧光是均匀的，但在较高的稀释度下，荧光趋于褪色。结论是，gZP具有与5H4抗血清相关的抗原表位，该抗原表位与精子-精子相互作用有关，并且可能是开发避孕疫苗的良好候选者。

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《Small Ruminant Research》 |2000年第2期|共8页
作者
Gautam Kaul; Gandhi KK; Kaul G;
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正文语种 eng
中图分类动物学;
关键词
glycoproteins; monoclonal antibodies; zona pellucida; inhibition; fluorescence; spermatozoa; binding; goats;

机译：糖蛋白;单克隆抗体;透明带;抑制;荧光;精子;结合;山羊;

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1. Inhibition of goat sperm-zona binding by monoclonal antibodies to a glycoprotein family (ZP4) of porcine zona pellucida. [J] . Gautam Kaul, Gandhi KK, Kaul G Small Ruminant Research . 2000,第2期

机译：单克隆抗体对猪透明带糖蛋白家族（ZP4）的单克隆抗体抑制山羊精子-zona结合。
2. Monoclonal antibodies as probes of the distribution of ZP-2, the major sulfated glycoprotein of the murine zona pellucida. [J] . I J East, J Dean Journal of cell biology . 1984,第3期

机译：单克隆抗体作为ZP-2分布的探针，ZP-2是鼠透明带的主要硫酸化糖蛋白。
3. Immunocontraception of captive exotic species: V. Prolonged antibody titers in Dall sheep (Ovis dalli dalli) and domestic goats (Capra hircus) immunized with porcine zona pellucida. [J] . Lyda R. O., Frank K. M., Wallace R., Journal of Zoo and Wildlife Medicine . 2013,第4Suppla1期

机译：圈养外来物种的免疫避孕：V.用猪透明带免疫接种的野绵羊（Ovis dalli dalli）和家山羊（Capra hircus）的抗体滴度延长。
4. Human p2-Glycoprotein I Binds To Endothelial Cells Through A Cluster of Lysihe Residues that Are Critical for Anionic Phospholipid Binding and Offers Epitopes for Anti- p2-Glycoprotein I Antibodies [C] . Del Papa N, Sheng YH, Raschi E, NATO Advanced Study Institute on Vascular Endothelium : Mechanisms of Cell Signaling . 1999

机译：人P2-糖蛋白I通过对阴离子磷脂结合至关重要的Lysihe残基团结合内皮细胞，并为抗P2-糖蛋白I抗体提供表位
5. The identification and characterization of an inner acrosomal membrane associated protein, IAM38, responsible for secondary sperm-zona binding during fertilization. [D] . Yu, Yang. 2009

机译：内顶体膜相关蛋白IAM38的鉴定和表征，该蛋白负责受精过程中的继发精子-精子结合。
6. Porcine sperm-zona binding ability as an indicator of fertility [O] . E. D. Collins, W. L. Flowers, R. D. Shanks, -1

机译：猪精子-zona结合能力是生育能力的指标
7. Oviduct-Specific Glycoprotein Modulates Sperm-Zona Binding and Improves Efficiency of Porcine Fertilization In Vitro1 [O] . T.C. McCauley, W.C. Buhi, G.M. Wu, 2003

机译：特异性糖蛋白调节精子 - Zona结合，提高Vitro1中猪施肥的效率
8. Monoclonal Antibodies Against Human LFA-1 Cross-React with Porcine LFA-1 and Inhibit Porcine T-Lymphocyte and Natural Killer Cell Function [R] . Hildreth, J. E., Holt, V., August, J. T., 1986

机译：针对人LFa-1的单克隆抗体与猪LFa-1交叉反应并抑制猪T淋巴细胞和自然杀伤细胞功能

Inhibition of goat sperm-zona binding by monoclonal antibodies to a glycoprotein family (ZP4) of porcine zona pellucida.

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