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首页> 外文期刊>Small Ruminant Research >Three novel MC4R SNPs associated with growth traits in Hu sheep and East Friesian x Hu crossbred sheep
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Three novel MC4R SNPs associated with growth traits in Hu sheep and East Friesian x Hu crossbred sheep

机译：三种与胡羊和东弗里斯兰×胡杂交羊生长性状相关的新型MC4R SNP

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The melanocortin-4 receptor (MC4R) is a G-protein coupled receptor superfamily member, which has been implicated in regulating food intake, energy balance and body weight in mammals. Though the mutations of MC4R have been identified as genetic markers for growth traits in many livestock species, little is known about sheep MC4R gene. In this study, MC4R gene was amplified and sequenced to screen single nucleotide polymorphism sites (SNPs) and three novel SNPs (g.306 G>A and g.706 C>A in the coding region and g.1267 G>A in the 3'-UTR) were detected. The genetic structure of the three SNPs were examined by PCR-RFLP and CRS-PCR-RFLP, respectively, in 298 individuals of two sheep populations (Hu sheep, Hu; East Friesian x Hu crossbred sheep, EH). Association analysis between three SNPs and body traits revealed that two of the SNPs were associated with body weight, except for g.306 G>A mutation; and all three loci were significantly associated with body size. Haplotype blocks showed that there were none loci linked within Hu nor EH MC4R gene, but both generated principally seven haplotypes. Moreover, H1H3 (GA-CC-GA) diplotype had better performance than other diplotypes in Hu sheep (P 0.05), while all of diplotypes had no significant difference in EH sheep (P > 0.05). These preliminary results indicate that three novel MC4R SNPs are related to the growth development, which may be used as genetic markers for sheep breeding and potentially afford a good foundation for further study on MC4R gene. (C) 2015 Elsevier B.V. All rights reserved.
机译：melanocortin-4受体（MC4R）是G蛋白偶联受体超家族成员，已参与调节哺乳动物的食物摄入，能量平衡和体重。尽管已将MC4R的突变鉴定为许多牲畜物种生长性状的遗传标记，但对绵羊MC4R基因了解甚少。在这项研究中，MC4R基因进行扩增和测序以筛选单核苷酸多态性位点（SNPs）和三个新的SNPs（g.306 G> A和g.706 C> A在编码区和g.1267 G> A在编码区。 3'-UTR）。通过PCR-RFLP和CRS-PCR-RFLP分别检查了两个绵羊种群（Hu绵羊，Hu; East Friesian x Hu杂交绵羊，EH）的298个个体中三个SNP的遗传结构。三个SNP与身体特征之间的关联分析表明，除了g.306 G> A突变外，两个SNP与体重有关。并且所有三个位点均与体型显着相关。单倍型模块显示，Hu和EH MC4R基因内没有连接的基因座，但均产生了7个单倍型。此外，H1H3（GA-CC-GA）双倍型在胡羊中的表现优于其他双倍型（P <0.05），而所有双倍型在EH羊中均无显着性差异（P> 0.05）。这些初步结果表明，三种新颖的MC4R SNP与生长发育有关，可以用作绵羊育种的遗传标记，并可能为进一步研究MC4R基因提供良好的基础。 （C）2015 Elsevier B.V.保留所有权利。

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