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首页> 外文期刊>Skin research and technology: official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) >Non-invasive assessment of healing of bacteria infected and uninfected wounds using optical coherence tomography.
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Non-invasive assessment of healing of bacteria infected and uninfected wounds using optical coherence tomography.

机译：使用光学相干层析成像技术对感染和未感染细菌的伤口愈合进行非侵入性评估。

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BACKGROUND/PURPOSE: Bacterial infection is one of the main predisposing factors for the delay in wound healing. To facilitate a timely decision for correct therapy, it is important to accurately monitor the morphological changes in the infected wounds using noninvasive tools. In the present study, we have explored the use of optical coherence tomography (OCT) for monitoring the healing of superficial wounds infected with Staphylococcus aureus in mice under in vitro and in vivo conditions and studied the changes in collagen birefringence in the infected wounds. METHODS: The tape stripping method was used for generating superficial skin wounds in mice and wounds were infected with S. aureus. For in vitro studies, infected and uninfected wound tissues were resected, back scattered intensity and birefringence changes in collagen during wound healing were studied on the 2, 4 and 10th day of postinfection using polarization-sensitive (PS) OCT and images were compared with histology. Real-time OCT was used for studying the kinetics of healing of infected wounds under in vivo conditions. RESULTS: From the PS-OCT images, different phases of wound healing such as inflammation, reepithelialization and collagen remodeling could be identified. The edematic regions appeared prominent in infected wounds. Compared with uninfected wounds, reepithelization and collagen remodeling phases of wound healing were delayed significantly in the infected wounds. These changes were comparable with the different stages of wound healing observed under in vivo conditions. CONCLUSION: OCT imaging can provide a rapid assessment of the morphological changes associated with bacteria-infected and uninfected wounds and thereby aid in timely treatment planning.
机译：背景/目的：细菌感染是导致伤口愈合延迟的主要诱因之一。为了便于及时决定正确的治疗方法，使用无创工具准确监测感染伤口的形态变化非常重要。在本研究中，我们探索了光学相干断层扫描（OCT）在体外和体内条件下监测小鼠感染金黄色葡萄球菌表浅伤口的愈合情况，并研究了胶原双折射在感染伤口中的变化。方法：用胶带剥离法在小鼠表面产生皮肤伤口，并用金黄色葡萄球菌感染伤口。在体外研究中，切除感染和未感染的伤口组织，在伤口愈合后的第2、4和10天使用偏光敏感（PS）OCT研究胶原蛋白的后向散射强度和双折射变化，并将图像与组织学进行比较。实时OCT用于研究体内条件下感染伤口愈合的动力学。结果：从PS-OCT图像中，可以确定伤口愈合的不同阶段，例如炎症，再上皮形成和胶原蛋白重塑。在感染的伤口中，水肿区域显得突出。与未感染的伤口相比，感染伤口的再上皮化和胶原蛋白重塑阶段明显延迟。这些变化与在体内条件下观察到的伤口愈合的不同阶段相当。结论：OCT成像可以快速评估与细菌感染和未感染伤口相关的形态变化，从而有助于及时制定治疗计划。

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