• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Skin research and technology: official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) >High-resolution multiphoton tomography and fluorescence lifetime imaging of UVB-induced cellular damage on cultured fibroblasts producing fibres.
【24h】

High-resolution multiphoton tomography and fluorescence lifetime imaging of UVB-induced cellular damage on cultured fibroblasts producing fibres.

机译：高分辨率多光子层析成像和UVB诱导培养的成纤维细胞产生的细胞损伤的荧光寿命成像。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Multiphoton tomography (MPT) is suitable to perform both ex vivo and in vivo investigations of living skin and cell cultures with submicron resolution. Fluorescence lifetime imaging (FLIM) generates image contrast between different states of tissue characterized by various fluorescence decay rates. Our purpose was to combine MPT and FLIM to evaluate fibroblasts and collagen fibres produced in vitro.Fibroblast cultures, 2-4 days old, at a subconfluent stage, were evaluated before and after irradiation with a single UVB dose. One month old cultures stimulated with ascorbic acid were also assessed.After UVB radiation, fibroblasts appear irregular in size, lose their alignment and show a decrease in fluorescence lifetime. One month-old fibroblasts, producing collagen fibres after stimulation with ascorbic acid, appear as small roundish structures intermingled by filaments showing a granular arrangement.The combination of MPT and FLIM may be useful for the in vitro study of cell modifications induced by injurious or protective agents and drugs.
机译：多光子断层扫描（MPT）适用于以亚微米分辨率对活体皮肤和细胞培养物进行离体和体内研究。荧光寿命成像（FLIM）在以各种荧光衰减率为特征的组织不同状态之间生成图像对比度。我们的目的是将MPT和FLIM结合起来评估体外产生的成纤维细胞和胶原纤维。在亚汇合阶段对2-4天大的成纤维细胞培养物进行单剂量UVB照射前后的评估。还评估了抗坏血酸刺激的一个月大的培养物.UVB辐射后，成纤维细胞的大小似乎不规则，失去排列并显示荧光寿命缩短。一个月大的成纤维细胞在抗坏血酸刺激后产生胶原纤维，表现为细小的圆形结构，被细丝混合呈颗粒状排列。MPT和FLIM的组合可用于体外研究伤害性或保护性诱导的细胞修饰代理商和毒品。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号