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首页> 外文期刊>Soil Biology & Biochemistry >Metal-induced oxidative stress impacting plant growth in contaminated soil is alleviated by microbial siderophores
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Metal-induced oxidative stress impacting plant growth in contaminated soil is alleviated by microbial siderophores

机译：金属诱导的氧化胁迫影响受污染土壤中植物的生长，可通过微生物铁载体缓解

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High levels of metals impede plant growth by affecting physiological processes. Siderophores are microbial Fe-chelators that, however, bind other metals. This study evaluated plant growth in a soil containing elevated levels of metals, including Al, Cu, Fe, Mn, Ni, and U, using Streptomyces-derived cell-free supernatant containing siderophores and auxins. Cowpea plants in the soil were treated with the culture filtrate. Growth was measured and biochemical analyses such as chlorophyll contents, RNA and protein quantification, lipid membrane peroxidation, and anti- oxidative responses were conducted to evaluate oxidative stress in the plants. Liquid chromatography-mass spectrometry was used to simulate competition for siderophore binding, and metal content of plants was determined spectroscopically. Whereas the metals inhibited plant growth, addition of siderophores improved growth. There was evidence of lipid peroxidation, an enhanced superoxide dismutase activity, and lowered chlorophyll, RNA, protein, carotenoid and residual indole acetic acid contents, especially in control plants. Siderophore competition assays between Al and Fe, and Fe and Cu suggested that trivalent metals are more competitive for siderophore binding than divalent ones. Compared to control plants, higher amounts of metals were obtained in siderophore-treated plants. Siderophores were able to supply plants with Fe in the presence of levels of metals, mainly Al, Cu, Mn, Ni and U that otherwise inhibit Fe acquisition. This led to enhanced chlorophyll content, circumventing lipid peroxidation effects on leaves. Siderophores lowered the formation of free radicals, thereby protecting microbial auxins from degradation and enabling them to enhance plant growth which in turn resulted in augmented metal uptake.
机译：高含量的金属通过影响生理过程而阻碍植物的生长。铁载体是微生物的铁螯合剂，但是与其他金属结合。这项研究使用链霉菌衍生的含有铁载体和生长素的无细胞上清液，评估了在含有高水平金属（包括Al，Cu，Fe，Mn，Ni和U）的土壤中的植物生长。用培养滤液处理土壤中的豆植物。测量生长并进行生化分析，例如叶绿素含量，RNA和蛋白质定量，脂质膜过氧化和抗氧化反应，以评估植物的氧化胁迫。用液相色谱-质谱法模拟铁载体的竞争，并用光谱法测定植物的金属含量。尽管这些金属抑制了植物的生长，但是添加铁载体可以促进植物的生长。有证据显示脂质过氧化，超氧化物歧化酶活性增强，叶绿素，RNA，蛋白质，类胡萝卜素和残留的吲哚乙酸含量降低，尤其是在对照植物中。 Al和Fe以及Fe和Cu之间的铁载体竞争分析表明，三价金属在铁载体结合方面比二价金属更具竞争性。与对照植物相比，在铁载体处理的植物中获得了更高量的金属。铁载体能够在一定水平的金属（主要是铝，铜，锰，镍和铀）存在下为植物提供铁，这些金属否则会抑制铁的获取。这导致叶绿素含量增加，从而规避了脂质对叶片的过氧化作用。铁载体降低了自由基的形成，从而保护了微生物生长素免于降解，并使它们能够促进植物生长，从而增加了金属的吸收。

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