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首页> 外文期刊>Soil Biology & Biochemistry >Fate of prions in soil: Degradation of recombinant prion in aqueous extracts from soil and casts of two earthworm species
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Fate of prions in soil: Degradation of recombinant prion in aqueous extracts from soil and casts of two earthworm species

机译：病毒在土壤中的命运：重组soil病毒在土壤中的cast提取物和两种earth的铸型中的降解

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摘要

Prions represent the active agent in transmissible spongiform encephalopathy (TSE) diseases and can remain infective to mammals even after prolonged periods in soil. The influence of mesofauna on prion dispersal and degradation in soil, however, remains unknown. In this study the effect of earthworms on the retention/dissemination of TSEs in soil was evaluated using a model recombinant prion protein (recPrP) and aqueous extracts from soil and fresh casts of two earthworm species, Lumbricus terrestris and Aporrectodea caliginosa. Our results showed that earthworm gut-derived enzymes did not enhance the degradation of recPrP in comparison to soil, even though non-prion related proteolytic activity was higher in fresh worm excrements than in soil samples. Complete degradation of recPrP occurred in the aqueous extracts from all samples within up to 6 days at +15 degree C. The proteolytic enzymes responsible for degrading recPrP were inhibited by aprotinin and leupeptin and studies in pure cultures suggested these were most probably of soil microbial origin.
机译：ions病毒是传染性海绵状脑病（TSE）疾病中的活性剂，即使在土壤中长期放置后，仍可以感染哺乳动物。中生动物对病毒在土壤中的扩散和降解的影响仍然未知。在这项研究中，使用模型重组病毒蛋白（recPrP）以及土壤和两种earth的新鲜粪便和Lu菜的含水提取物评估worm对TSE在土壤中的保留/传播的影响。我们的结果表明，尽管新鲜蠕虫粪便中与enzyme病毒无关的蛋白水解活性高于土壤样品，但earth肠源性酶与土壤相比并没有促进recPrP的降解。在+15摄氏度下，最多6天之内，所有样品的水提物中都发生了recPrP的完全降解。抑肽酶和亮肽素抑制了降解recPrP的蛋白水解酶，在纯培养物中的研究表明，这些蛋白很可能是土壤微生物来源。

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