...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Soil Biology & Biochemistry >Liquid extraction of low molecular mass organic acids and hydroxamate siderophores from boreal forest soil.
【24h】

Liquid extraction of low molecular mass organic acids and hydroxamate siderophores from boreal forest soil.

机译：从北方森林土壤中液体萃取低分子量有机酸和异羟肟酸酯铁载体。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Low molecular mass organic acids (LMMOAs) and hydroxamate siderophores (HS) are molecules secreted by microbes and have previously been found in soil solution and in cultures. Mycorrhizal fungi are suggested to be involved in the nutrient uptake processes of trees and weathering of minerals. In this study soil samples taken from the O and E horizons of a podzol were extracted with 10 mM potassium phosphate buffer at pH 7.2. Variable parameters included addition of methanol to the extraction buffer and the use of ultrasonication or rotary shaking during extraction. LMMOAs and HS content of the soil extracts were determined. Analysis of soil extracts were carried out by liquid chromatography mass spectrometry (LC-MS) and the extraction results compared to results for soil solution samples obtained by centrifugation of the soils sampled. The extraction yields were significantly increased by addition of methanol to the extraction buffer, especially for the O horizon samples. Rotary shaking of the samples for 90 min gave slightly higher yields than ultrasonication for 15 min but the reduction in extraction time makes ultrasonication an attractive option. Of the HSs determined, ferricrocin was found in all samples. Optimal extraction conditions showed citric acid and isocitric acid to be the most abundant organic acids in the O and E horizons, respectively.
机译：低分子量有机酸（LMMOA）和异羟肟酸酯铁盐（HS）是微生物分泌的分子，以前已在土壤溶液和培养物中发现。建议菌根真菌参与树木的养分吸收过程和矿物质的风化。在这项研究中，使用pH 7.2的10 mM磷酸钾缓冲液提取了从Podzol的O和E层采集的土壤样品。可变参数包括向提取缓冲液中添加甲醇，以及在提取过程中使用超声或旋转摇动。测定土壤提取物中的LMMOA和HS含量。土壤提取物的分析通过液相色谱质谱法（LC-MS）进行，并将提取结果与通过离心取样的土壤获得的土壤溶液样品的结果进行比较。通过向提取缓冲液中添加甲醇，可以显着提高提取产率，尤其是对于O地平线样品而言。样品进行90分钟的旋转摇动比15分钟的超声处理产生的收率略高，但是减少的提取时间使超声处理成为有吸引力的选择。在确定的HS中，在所有样品中均发现了铁铁蛋白。最佳提取条件表明，柠檬酸和异柠檬酸分别是O和E层中最丰富的有机酸。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号