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首页> 外文期刊>Soil Biology & Biochemistry >Effect of calco-magnesian amendment on the mineral weathering abilities of bacterial communities in acidic and silicate-rich soils
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Effect of calco-magnesian amendment on the mineral weathering abilities of bacterial communities in acidic and silicate-rich soils

机译:钙镁改良剂对酸性和富含硅酸盐土壤中细菌群落矿物风化能力的影响

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Liming of forest ecosystem is recognized to increase nutrients availability in soil and water and to enhance the biomass and bacterial activities in soil. However, little studies have investigated on change induced on bacterial ability to alter silicate mineral after soil liming. Thus, this study is carried out in experimental conditions using podzol collected in the winter (2007), spring and summer (2008) in small forest catchments in Vosges Mountains amended in 2003 by dolostone and limestone. Bacterial communities, extracted from various horizons of these soils amended or not, were put in contact with a phyllosilicate (phlogopite as sole source of Mg and Fe) in miniaturized bioassays in aerobic conditions. A weathering phenotype was determined through the quantification of (i) protons and organic acids released in assay solution by bacteria (ii) iron leached from phlogopite lattice into solution by bacteria and (iii) the carbon source consumption (i.e. glucose). These results were then compared to empirical model based on chemical leaching experiments realized in the same conditions in order to simulate the processes involved. In parallel, the carbon source utilization patterns of bacteria were investigated in order to discriminate the bacterial communities from amended and non-amended soil horizons. The results indicate that (1) the total bacterial biomass was unaffected by the Ca-Mg amendment, whereas the cultivable bacterial biomass increased after the amendment, and (2) the weathering and the carbon source utilization patterns of bacterial communities differs from one soil horizon to another and among soil types. The metabolic profiles analyzed indicated significant differences in organic C substrate usage depending on season and Ca-Mg amendment. Bacterial communities, extracted from the amended soil, are enable greater iron leaching compared to those found in the control soil, suggesting a greater release of organic acids and/or a more highly chelating organic acid release. The process developed by bacteria to alter the phillosilicate is complexolysis. We conclude that the Ca-Mg amendment had a positive effect on the functional richness of bacterial communities extracted from soil and on their potential to weather minerals that was present after several years. (C) 2012 Elsevier Ltd. All rights reserved.
机译:森林生态系统的石灰化被认为可以增加土壤和水中的养分利用率,并增强土壤中的生物量和细菌活性。但是,很少有研究调查土壤定殖后细菌改变硅酸盐矿物的能力引起的变化。因此,这项研究是在实验条件下使用冬季(2007年),春季和夏季(2008年)在孚日山脉小森林集水区(2003年由白云岩和石灰石修订)中采集的podzol进行的。在有氧条件下的小型生物测定中,将从这些土壤的各个视野中提取或未修改的细菌群落与页硅酸盐(金云母作为镁和铁的唯一来源)接触。通过定量(i)细菌在测定溶液中释放的质子和有机酸(ii)细菌从金云母晶格浸出到溶液中的铁和(iii)碳源消耗(即葡萄糖)来确定风化表型。然后将这些结果与基于在相同条件下实现的化学浸出实验的经验模型进行比较,以模拟所涉及的过程。同时,研究了细菌的碳源利用模式,以便将细菌群落与改良土壤层和非改良土壤层区分开。结果表明:(1)Ca-Mg改良剂对总细菌生物量没有影响,而修正后可培养的细菌生物量却增加了;(2)细菌群落的风化和碳源利用方式与一个土壤层不同。到另一种土壤之间。分析的代谢曲线表明,根据季节和钙镁含量的变化,有机碳底物的使用量存在显着差异。与在对照土壤中发现的细菌群落相比,从改良土壤中提取的细菌群落能够实现更多的铁浸出,这表明有机酸的释放量更大和/或螯合性更高的有机酸释放量更高。细菌开发的改变硅硅酸盐的过程是复合分解。我们得出的结论是,Ca-Mg修正剂对从土壤中提取的细菌群落的功能丰富度及其几年后存在的矿物风化潜力具有积极影响。 (C)2012 Elsevier Ltd.保留所有权利。

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