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Evaluation of direct and indirect phosphorus limitation of methanogenic pathways in a calcareous subtropical wetland soil

机译:石灰性亚热带湿地土壤中产甲烷途径的直接和间接磷限制的评估

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摘要

The effect of phosphorus (P) and carbon (C) on methanogenesis was investigated in a low-P (130 mg P kg(-1) soil) wetland within Everglades National Park. Soil was amended with C substrates (acetate, formate, butyrate, and glucose) with or without P, and CO2 and CH4 production was monitored. Production of CH4 increased with P addition although no effect on CO2 was observed. Methane production was stimulated by all C substrates except for butyrate. No effect of C on CO2 production was observed except for stimulation following glucose addition. Production of CH4 following formate addition was not affected by P, suggesting hydrogenotrophic methanogens may be substrate, not P, limited. Addition of P to all other C substrates heightened CH4 production and lowered the CO2-C:CH4-C ratio relative to the corresponding C only treatment, suggesting that P may have limited acetoclastic methanogens and fermentation
机译:在大沼泽国家公园内的低磷(130 mg P kg(-1)土壤)湿地中研究了磷(P)和碳(C)对甲烷生成的影响。用含或不含P的C底物(乙酸,甲酸,丁酸和葡萄糖)修正土壤,并监测CO2和CH4的产生。尽管未观察到对CO2的影响,但随着P的添加,CH4的产量增加了。除丁酸酯外,所有C底物均会刺激甲烷的产生。除了添加葡萄糖后的刺激之外,未观察到C对CO 2产生的影响。添加甲酸后CH4的产生不受P的影响,表明氢营养型产甲烷菌可能是受限制的底物,而不是P。与所有仅C处理相比,将P添加到所有其他C底物上会提高CH4的产生并降低CO2-C:CH4-C的比率,这表明P可能具有有限的破乳产甲烷菌和发酵作用

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