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首页> 外文期刊>Soil Biology & Biochemistry >Molecular techniques and stable isotope ratios at natural abundance give complementary inferences about N2O production pathways in an agricultural soil following a rainfall event
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Molecular techniques and stable isotope ratios at natural abundance give complementary inferences about N2O production pathways in an agricultural soil following a rainfall event

机译：天然丰度下的分子技术和稳定的同位素比给出了降雨事件后农业土壤中N2O产生途径的补充推论

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摘要

The abatement of anthropogenic nitrous oxide (N2O) emissions depends on sound management of manure nitrogen (N) and an ability to track and predict manure-derived microbial N2O production in soils. The objective of this study was to investigate the utility of applying a novel combination of micrometeorological, stable isotope, and molecular methods to determine the short-term dynamics of N2O production processes in soil. Nitrous oxide emissions were continuously monitored in two drought-stressed agricultural fields treated with liquid dairy manure applied either in the fall or spring over an N2O emission event triggered by a heavy rainfall. In situ delta N-15-N2O and delta O-18-N2O measurements were used in conjunction with abundance (DNA) and potential activity (cDNA) measurements of key microbial N cycling gene and transcript (mRNA) targets to evaluate if these two techniques provided similar inferences about N2O soil processes occurring over the emission event. Soil gas was sampled at 4 depths along a profile from 10 to 50 cm below the surface and a multi-layer diffusion model was used to calculate the vertical N2O fluxes, the change in storage, and the net production of N2O in each layer.
机译：人为产生的一氧化二氮（N2O）排放的减少取决于粪便氮（N）的合理管理以及跟踪和预测土壤中粪便衍生的微生物N2O产生的能力。这项研究的目的是研究应用微气象学，稳定同位素和分子方法的新颖组合来确定土壤中N2O生产过程的短期动态的实用性。在秋季或春季由于暴雨引发的N2O排放事件中，使用液态乳牛粪处理的两个干旱胁迫农田中，持续监测一氧化二氮的排放。使用原位增量N-15-N2O和增量O-18-N2O测量与关键微生物N循环基因和转录本（mRNA）靶标的丰度（DNA）和潜在活性（cDNA）测量相结合，以评估这两种技术是否有效对排放事件中发生的N2O土壤过程提供了类似的推论。沿地面以下10至50 cm的剖面在4个深度处采样土壤气体，并使用多层扩散模型计算垂直N2O通量，储量变化和每层N2O的净产量。

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