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首页> 外文期刊>Signal transduction: Receptors, mediators and genes: Official journal of the Signal Transduction Society >Characterisation of lipids in cell signalling and membrane dynamics by nuclear magnetic resonance spectroscopy and mass spectrometry
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Characterisation of lipids in cell signalling and membrane dynamics by nuclear magnetic resonance spectroscopy and mass spectrometry

机译：核磁共振波谱和质谱法表征细胞信号传导和膜动力学中的脂质

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摘要

Signalling lipids regulate cellular events directly by recruiting and/or activating specific proteins or indirectly by locally modifying the physical properties of the membrane. These events trigger the co-localisation and interaction of proteins on membranes. This in turn effects the function and the properties of the proteins. To enable a comprehensive study of the complex and spatially heterogeneous natural membrane, complementary analytical tools need to be applied. Spectroscopy tools such as nuclear magnetic resonance spectroscopy and mass spectrometry provide the sensitivity and the precision required for detecting low amounts of highly transient signalling lipids, but also determine their effect on membrane structure. Specific examples are used to illustrate the application of liquid NMR spectroscopy, solid state NMR spectroscopy and electrospray ionisation mass spectrometry. The main advantages and limitations of these tools are discussed in this review.
机译：信号脂质通过募集和/或激活特定蛋白直接调节细胞事件，或通过局部修饰膜的物理特性间接调节细胞事件。这些事件触发了蛋白质在膜上的共定位和相互作用。这进而影响蛋白质的功能和特性。为了全面研究复杂的空间异质天然膜，需要使用互补的分析工具。诸如核磁共振波谱法和质谱法之类的光谱学工具可提供检测少量高瞬态信号脂质所需的灵敏度和精度，同时还可确定其对膜结构的影响。具体实施例用于说明液体NMR光谱，固态NMR光谱和电喷雾电离质谱的应用。本文将讨论这些工具的主要优点和局限性。

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