Heat Stress-Dependent DNA Binding of Arabidopsis Heat Shock Transcription Factor HSF1 to Heat Shock Gene Promoters in Arabidopsis Suspension Culture Cells in vivo

Lemin Zhang; Christian Lohmann; Ralf Prandl; Friedrich Schoffl

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Heat Stress-Dependent DNA Binding of Arabidopsis Heat Shock Transcription Factor HSF1 to Heat Shock Gene Promoters in Arabidopsis Suspension Culture Cells in vivo

机译：拟南芥悬浮培养细胞中拟南芥热休克转录因子HSF1对热休克基因启动子的热应激依赖性DNA结合。

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Using UV laser cross-linking and immunoprecipitation we measured the in vivo binding of Arabidopsis heat shock transcription factor HSF1 to the promoters of target genes, Hsp18.2 and Hsp70. The amplification of promoter sequences, co-precipitated with HSF1-specific antibodies, indicated that HSF1 is not bound in the absence of heat stress. Binding to promoter sequences of target genes is rapidly induced by heat stress, continues throughout the heat treatment, and declines during subsequent recovery at room temperature. The molecular mechanisms underlying the differences between Hsp18.2 and Hsp70 in the kinetics of HSF1/promoter binding and corresponding mRNA expression profiles are discussed.

机译：使用紫外线激光交联和免疫沉淀，我们测量了拟南芥热休克转录因子HSF1与靶基因Hsp18.2和Hsp70启动子的体内结合。与HSF1特异性抗体共沉淀的启动子序列的扩增表明，在不存在热应激的情况下，HSF1没有结合。通过热应激迅速诱导与靶基因的启动子序列的结合，在整个热处理过程中持续结合，并在随后的室温下恢复期间下降。讨论了Hsp1 /启动子结合动力学和相应的mRNA表达谱中Hsp18.2和Hsp70之间差异的分子机制。

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《Biological chemistry》 |2003年第6期|共5页
作者
Lemin Zhang; Christian Lohmann; Ralf Prandl; Friedrich Schoffl;
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正文语种 eng
中图分类生物化学;
关键词
Hsp18.2; Hsp70; immunoprecipitation; mRNA profiling; real-time; stress response;

机译：Hsp18.2;Hsp70;免疫沉淀;mRNA分析;实时;应激反应;

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专利

1. Heat Stress-Dependent DNA Binding of Arabidopsis Heat Shock Transcription Factor HSF1 to Heat Shock Gene Promoters in Arabidopsis Suspension Culture Cells in vivo [J] . Lemin Zhang, Christian Lohmann, Ralf Prandl, Biological chemistry . 2003,第6期

机译：拟南芥悬浮培养细胞中拟南芥热休克转录因子HSF1对热休克基因启动子的热应激依赖性DNA结合。
2. Phosphorylation by a cyclin-dependent kinase modulates DNA binding of the Arabidopsis heat-shock transcription factor HSF1 in vitro. [J] . Reindl A, Schffl F, Schell J, Plant physiology . 1997,第1期

机译：细胞周期蛋白依赖性激酶的磷酸化作用在体外调节拟南芥热休克转录因子HSF1的DNA结合。
3. Interaction between the Arabidopsis thaliana heat shock transcription factor HSF1 and the TATA binding protein TBP [J] . Reindl Andreas, Schouml, ffl Fritz FEBS Letters . 1998,第3期

机译：拟南芥热激转录因子HSF1与TATA结合蛋白TBP的相互作用
4. Mining Heat Stress Induced Genes Encoding Heat Shock Proteins (HSPs) and Heat Stress Transcription Factors (HSFs) in Strawberry (Fragaria x ananassa) [C] . Lee-Fong Lin, Shih-Yi Liao, Jui-Hung Yen, International Conference on Mechanics and Control Engineering . 2014

机译：采矿热应激诱导在草莓（Fragaria x ananassa）中编码热休克蛋白（HSP）和热应激转录因子（HSF）的热应激诱导基因
5. Regulation of heat shock factor 1 (HSF1) DNA -binding and transcription [D] . Mercier, Philippe Arthur 2003

机译：调节热激因子1（HSF1）DNA结合和转录
6. The Natural Compound Cantharidin Induces Cancer Cell Death through Inhibition of Heat Shock Protein 70 (HSP70) and Bcl-2-associated Athanogene Domain 3 (BAG3) Expression by Blocking Heat Shock Factor 1 (HSF1) Binding to Promoters [O] . Joo Ae Kim, Youngmi Kim, Byoung-Mog Kwon, 2013

机译：天然化合物斑th素通过抑制热休克因子1（HSF1）与启动子的结合通过抑制热休克蛋白70（HSP70）和与Bcl-2相关的致癌基因域3（BAG3）的表达诱导癌细胞死亡。
7. Phosphorylation by a cyclin-dependent kinase modulates DNA binding of the Arabidopsis heat-shock transcription factor HSF1 in vitro. [O] . Reindl, A, Schöffl, F, Schell, J, 1997

机译：细胞周期蛋白依赖性激酶的磷酸化在体外调节拟南芥热休克转录因子HSF1的DNA结合。

Heat Stress-Dependent DNA Binding of Arabidopsis Heat Shock Transcription Factor HSF1 to Heat Shock Gene Promoters in Arabidopsis Suspension Culture Cells in vivo

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