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首页> 外文期刊>South African Journal of Botany >In vitro regeneration of the Kalahari devil's claw, Harpagophytumprocumbens, an important medicinal plant
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In vitro regeneration of the Kalahari devil's claw, Harpagophytumprocumbens, an important medicinal plant

机译：重要药用植物卡拉哈里魔爪Harpagophytumprocumbens的体外再生

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摘要

Harpagophytum procumbens DC (Pedaliaceae), also known as the Kalahari devil's claw, is an important medicinal plant in southern Africa. An in vitro regeneration protocol has been achieved via shoot tip cultures. Single node segments and shoot tips were cultured on Murashige and Skoog medium supplemented with various BA concentrations in combination with NAA. All media tested induced axillary buds, shoot tip growth and root formation. Shoot length decreased with an increase in BA concentration. Maximal shoot length was obtained in MS medium with no hormone supplement, and average shoot number ranged between 1.5-2.2 shoots/explant. Addition of NAA improved growth of the cultures but induced callus formation.
机译：Harpagophytum procumbens DC（Pedaliaceae），也被称为卡拉哈里魔爪，是南部非洲的重要药用植物。通过芽尖培养已经实现了体外再生方案。在补充了各种BA浓度和NAA的Murashige和Skoog培养基上培养单节节段和茎尖。测试的所有培养基均会引起腋芽，枝梢生长和根形成。随着BA浓度的增加，芽的长度减少。在不添加激素的MS培养基中获得最大芽长，平均芽数在1.5-2.2个芽/植株之间。 NAA的添加改善了培养物的生长，但是诱导了愈伤组织的形成。

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