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Effects of endophytic actinomycetes and Bradyrhizobium japonicum strains on growth, nodulation, nitrogen fixation and seed weight of different soybean varieties.

机译:内生放线菌和日本大豆根瘤菌菌株对不同大豆品种生长,结瘤,固氮和种子重量的影响。

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Effects of endophytic actinomycetes and two Bradyrhizobium japonicum (Kirchner 1896) Jordan 1982 strains on growth, nodulation, nitrogen (N) fixation and seed weight of different soybean (Glycine max L. Merr.) varieties from Myanmar (Hinthada), Thailand (SJ5) and Cambodia (DT84) were studied in pot experiments in Thailand. The six treatments consisted of an uninoculated control (T1), a single inoculation with endophytic actinomycetes (Streptomyces sp. strain, P4) (T2), a single inoculation with Bradyrhizobium japonicum strains, USDA110 (T3) or THA7 (T4) and a dual inoculation of P4 with USDA110 (T5) or THA7 (T6). Sterile soil was used for cultivation of all treatments under open field conditions, using tap water for irrigation. N2 fixation activity of soybean was evaluated by the relative ureide method using xylem solute from the root bleeding sap at the early pod-fill stage. It was found that the single inoculation of P4 did not show significant effects on nodulation, N2 fixation, shoot dry weight and seed weight at harvest of all soybeans compared with the uninoculated control. In the Hinthada soybean, single inoculation of USDA110 or THA7 increased seed weight while USDA110 improved N2 fixation (P<0.05). Dual inoculation of USDA110 and P4 showed the highest shoot N accumulation and seed weight among treatments. In the SJ5 soybean, similar trends to Hinthada were shown, although the THA7 and P4 combination showed the highest shoot N accumulation and seed weight. On the other hand, in the DT84 soybean, there were no significant differences of N2 fixation and seed weight between USDA110 and THA7 (both single and dual inoculation). In conclusion, dual inoculation of endophytic actinomycetes strain P4 may increase the nodulation and nitrogen fixation in some varieties; however, it is not effective for other varieties.Digital Object Identifier http://dx.doi.org/10.1080/00380768.2012.682044
机译:内生放线菌和两种日本气生根瘤菌(Kirchner 1896)Jordan 1982菌株对不同大豆(Glycine max L.)生长,结瘤,固氮和种子重量的影响。在泰国的盆栽试验中研究了来自缅甸(欣达达),泰国(SJ5)和柬埔寨(DT84)的Merr。)品种。这六种治疗方法包括未接种的对照(T1),单次接种内生放线菌( Streptomyces 菌株,P4)(T2),单次接种 Bradyrhizobium japonicum 菌株USDA110(T3)或THA7(T4),并用USDA110(T5)或THA7(T6)双重接种P4。无菌土壤用于在露天条件下使用自来水灌溉的所有处理方法。在豆荚灌浆初期,采用根腐树液木质部溶质通过相对脲离子法评价大豆的N 2 固定活性。结果发现,与未接种对照相比,单次接种P4对所有大豆的结瘤,N 2 固定,芽干重和种子重均无显着影响。在Hinthada大豆中,单次接种USDA110或THA7会增加种子重量,而USDA110会改善N 2 固定( P <0.05)。在处理中,USDA110和P4的双重接种显示出最高的芽N积累和种子重量。在SJ5大豆中,显示出与Hinthada相似的趋势,尽管THA7和P4组合显示出最高的芽N积累和种子重量。另一方面,在DT84大豆中,USDA110和THA7(单接种和双接种)的N 2 固定和种子重量没有显着差异。总之,内生放线菌菌株P4的双重接种可能会增加某些品种的结瘤和固氮作用。但是,它对其他品种无效。数字对象标识符http://dx.doi.org/10.1080/00380768.2012.682044

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