• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>South African Journal of Wildlife Research >Detection and genetic analysis of feline immunodeficiency virus (FIVple) in southern African lions (Panthera leo)
【24h】

Detection and genetic analysis of feline immunodeficiency virus (FIVple) in southern African lions (Panthera leo)

机译：非洲南部狮子（Panthera leo）的猫免疫缺陷病毒（FIVple）的检测和遗传分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Feline immunodeficiency virus is a retrovirus of domestic cats that causes immuno-suppressive disease and lifelong infection. Lentivirus has also been detected in African lions (Panthera leo). The lentivirus infecting lions in southern Africa has never been isolated; thus, knowledge about its molecular characteristics in these populations is limited. Our investigation used whole blood samples collected opportunistically from free-ranging southern African lions in Kruger National Park, South Africa, and from Hlane Royal National Park in Swaziland to analyse the lentivirus. Whole blood samples from captive exotic felids from zoos in South Africa and the United States, and from domestic cats in South Africa were analysed for comparison. A nested polymerase chain reaction (PCR) assay was used to amplify a portion of the proviral DNA encoding the reverse transcriptase. The nucleotide sequence of all products was determined and examined. The PCR assay was successful in amplifying lion lentivirus, with 52 positive and 65 negative samples. Of the 34 sequences amplified from a variety of felids, six showed an average of 96% homology to domestic feline lentivirus, and 28 showed an average of 94% homology to lion lentivirus. In addition, domestic cat lentivirus nucleic acid was amplified from a captive tiger, demonstrating the possibility of cross-species transmission.
机译：猫免疫缺陷病毒是家猫的逆转录病毒，可引起免疫抑制疾病和终生感染。在非洲狮子（Panthera leo）中也发现了慢病毒。南部非洲从未感染过慢病毒的狮子。因此，在这些人群中关于其分子特性的知识是有限的。我们的研究使用了机会性采集的全血样本，这些样本是从南非克鲁格国家公园的自由放养的南非狮子以及斯威士兰的赫兰皇家国家公园采集的全血样本来分析的。对来自南非和美国动物园的圈养外来猫科动物以及南非家猫的全血样品进行了分析，以进行比较。巢式聚合酶链反应（PCR）测定法用于扩增编码逆转录酶的原病毒DNA的一部分。确定并检查所有产物的核苷酸序列。 PCR检测成功扩增了52例阳性和65例阴性样本的狮子慢病毒。从各种猫科动物扩增的34个序列中，有6个与家猫慢病毒的平均同源性为96％，有28个与狮子慢病毒的平均同源性为94％。此外，从圈养的老虎中扩增了家猫慢病毒核酸，证明了跨物种传播的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号