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首页> 外文期刊>Soil Science and Plant Nutrition >Cytosolic glutamine synthetase is present in the phloem sap of rice (Oryza sativa L.)
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Cytosolic glutamine synthetase is present in the phloem sap of rice (Oryza sativa L.)

机译：水稻（Oryza sativa L.）的韧皮部汁液中存在胞质谷氨酰胺合成酶

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摘要

The presence of glutamine synthetase (GS) in the rice sieve tube was examined. Proteins in the rice phloem sap from leaf sheaths were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis, transferred to polyvinylidine difluoride membranes and immunoblotted with anti-GS1 antibody. A cross-reacting band, thought to be GS1, was detected in the phloem sap. Moreover, the phloem sap contained a significant amount of GS transferase activity. Previous studies have shown that the concentrations of substrates and cofactors in the rice phloem sap are sufficient for cytosolic GS reaction. These data suggest that physiologically active GS1 is present in rice phloem sap, which might convert glutamate to glutamine in vivo.
机译：检查了米筛管中谷氨酰胺合成酶（GS）的存在。叶鞘中的水稻韧皮部汁液中的蛋白质通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离，转移至聚偏二氟乙烯膜上，并用抗GS1抗体进行免疫印迹。在韧皮部汁液中发现了一个交叉反应带，被认为是GS1。此外，韧皮部汁液含有大量的GS转移酶活性。以前的研究表明，水稻韧皮部汁液中底物和辅因子的浓度足以进行细胞质GS反应。这些数据表明，在水稻韧皮部汁液中存在生理活性的GS1，其可能在体内将谷氨酸转化为谷氨酰胺。

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