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首页> 外文期刊>Soil Science and Plant Nutrition >Detection of protease genes in field soil applied with liquid livestock feces and speculation on their function and origin
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Detection of protease genes in field soil applied with liquid livestock feces and speculation on their function and origin

机译：液态牲畜粪便在田间土壤中蛋白酶基因的检测及其功能和起源的推测

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摘要

The origin of soil protease in field soil was estimated using culture-dependent and independent approaches. Overall soil protease activity was much higher in field soils with an annual application of liquid livestock feces (120 t ha(-1) year(-1) and 600 t ha(-1) year(-1)) compared with the activity recorded in other field soils, and the character of the soil proteases became highly homogeneous (approximately 70% metalloprotease in a 600 t field). Selective incubation studies suggested that bacteria were the most important source of soil protease. There were significantly higher correlations between serratial metalloprotease and the overall soil protease in both feces-applied fields in terms of the effect of inhibitors, and the bacteria, which produced serratial metalloprotease, were suggested to proliferate in both the 120 t and 600 t fields. The gene homologous to serratial metalloprotease gene was amplified in directly extracted DNA from field soils using selective DNA primer and proteolytic Serratia marcescens was certified to be one source of soil protease in these field soils. Proteolytic S. marcescens and its metalloprotease gene have occasionally been isolated and detected in field soils applied with raw feces, and have rarely been isolated or detected from other field soils. Proteolytic S. marcescens is believed to be introduced in the raw feces and subsequently colonizes the field soil and replaces the indigenous bacteria in the soil.
机译：田间土壤中土壤蛋白酶的起源是使用依赖于培养的方法和独立的方法进行估算的。与记录的活性相比，使用液态牲畜粪便（120 t ha（-1）年（-1）年和600 t ha（-1）年（-1））每年施用的田间土壤中的总土壤蛋白酶活性高得多。在其他田间土壤中，土壤蛋白酶的特性变得高度均一（在600吨田中约70％的金属蛋白酶）。选择性温育研究表明细菌是土壤蛋白酶的最重要来源。就抑制剂的作用而言，在两个粪便施用的田地中，土壤金属蛋白酶与土壤中的总蛋白酶之间的相关性显着更高，并且建议产生土壤金属蛋白酶的细菌在120 t和600 t田地均繁殖。使用选择性DNA引物在直接从田间土壤中提取的DNA中扩增了与锯缘金属蛋白酶基因同源的基因，并且蛋白水解粘质沙雷氏菌被证明是这些田间土壤中的一种蛋白酶来源。偶尔会在施用生粪的田间土壤中分离和检测到蛋白水解链霉菌及其金属蛋白酶基因，很少从其他田间土壤中分离或检测到该蛋白。据认为，蛋白水解链霉菌被引入生粪中，随后在田间土壤中定殖并替代土壤中的原生细菌。

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