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首页> 外文期刊>Spine >Altered disc mechanics in mice genetically engineered for reduced type I collagen.
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Altered disc mechanics in mice genetically engineered for reduced type I collagen.

机译：为减少I型胶原蛋白而基因工程改造的小鼠的椎间盘机械改变。

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STUDY DESIGN: Mechanically test lumbar discs of transgenic mice in compression-tension and torsion. OBJECTIVES: Determine if a reduction in type I collagen results in decreased disc mechanics. SUMMARY OF BACKGROUND DATA: Quantitative relationships between disc structure and function would improve the understanding of disc generation and are essential relationships for functional tissue engineering. The reduced type I collagen transgenic mouse has been used in structure-function studies of bone and tendon, but not intervertebral discs. Methods for testing mouse discs have recently been developed, making disc structure-function studies possible. METHODS: Microradiographed and mechanically tested lumbar discs from control and collagen-reduced mice in both compression-tension and torsion were used. Disc area and polar moment of inertia were determined from radiographic data, stiffness from mechanical data, and apparent modulus from geometric and mechanical data. RESULTS: Collagen-reduced discs had a largerarea and polar moment of inertia compared to controls. The linear and torsional stiffness of collagen-reduced and control discs were not significantly different. Finally, the apparent modulus of collagen-reduced discs was significantly less than controls in compression (73% of control) and torsion (50%). CONCLUSIONS: Compared to controls, collagen-reduced discs had reduced apparent modulus in both loading directions, suggesting that the transgenic disc tissue was mechanically inferior to controls. These results are consistent with the widely accepted functional role of type I collagen in disc mechanics, and therefore supports the use of transgenic mice to study structure-function relationships of the disc. Future work will focus on quantifying structure-function relationships related to degeneration, as well as those relevant to the design of tissue-engineered disc replacements.
机译：研究设计：机械测试转基因小鼠的腰椎间盘在压缩-拉伸和扭转状态。目的：确定I型胶原蛋白的减少是否会导致椎间盘力学下降。背景数据概述：椎间盘结构与功能之间的定量关系将增进对椎间盘生成的理解，并且是功能组织工程学的基本关系。还原型I型胶原转基因小鼠已用于骨骼和肌腱的结构功能研究，但未用于椎间盘。最近已经开发了用于测试鼠标光盘的方法，从而使光盘结构功能研究成为可能。方法：对受压和胶原蛋白还原的小鼠的腰椎间盘进行放射线照相和机械测试，并得出其压缩压力和扭转力。圆盘面积和极惯性矩由射线照相数据确定，刚度由机械数据确定，表观模量由几何和机械数据确定。结果：与对照组相比，减少胶原蛋白的椎间盘具有更大的面积和极地惯性矩。减少胶原蛋白的椎间盘和对照椎间盘的线性和扭转刚度没有显着差异。最后，胶原蛋白减少的椎间盘的表观模量显着小于压缩（73％）和扭转（50％）。结论：与对照组相比，减少胶原蛋白的椎间盘在两个加载方向上的表观模量均降低，这表明转基因椎间盘的机械性能不如对照组。这些结果与椎间盘力学中I型胶原蛋白的功能作用一致，因此支持转基因小鼠研究椎间盘的结构功能关系。未来的工作将集中于量化与变性相关的结构-功能关系，以及与组织工程性椎间盘置换物的设计相关的结构-功能关系。

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