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首页> 外文期刊>Biomaterials >The use of chitosan to damage Cryptococcus neoformans biofilms.
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The use of chitosan to damage Cryptococcus neoformans biofilms.

机译：使用壳聚糖破坏新型隐球菌生物膜。

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摘要

The use of indwelling medical devices (e.g. pacemakers, prosthetic joints, catheters, etc) continues to increase, yet these devices are all too often complicated by infections with biofilm-forming microbes with increased resistance to antimicrobial agents and host defense mechanisms. We investigated the ability of chitosan, a polymer isolated from crustacean exoskeletons, to damage biofilms formed by the pathogenic fungus Cryptococcus neoformans. Using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium-hydroxide (XTT) reduction assay and CFU determinations, we showed that chitosan significantly reduced both the metabolic activity of the biofilms and cell viability, respectively. We further demonstrated that chitosan penetrated biofilms and damaged fungal cells using confocal and scanning electron microscopy. Notably, melanization, an important virulence determinant of C. neoformans, did not protect cryptococcal biofilms against chitosan. The chitosan concentrations used in this study to evaluate fungal biofilm susceptibility were not toxic to human endothelial cells. Our results indicate that cryptococcal biofilms are susceptible to treatment with chitosan, suggesting an option for the prevention or treatment of fungal biofilms on indwelling medical devices.
机译：留置医疗设备（例如起搏器，假肢关节，导管等）的使用仍在继续增加，但是这些设备常常因生物膜形成微生物的感染而变得更加复杂，这些微生物对抗菌剂和宿主防御机制的抵抗力有所增强。我们调查了壳聚糖（一种从甲壳类外骨骼中分离的聚合物）破坏由致病性真菌新隐球菌形成的生物膜的能力。使用2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-5-[（苯氨基）羰基] -2H-氢氧化四唑（XTT）还原测定法和CFU测定，我们表明壳聚糖可显着降低两者生物膜的代谢活性和细胞活力。我们进一步证明，使用共聚焦和扫描电子显微镜，壳聚糖穿透生物膜并破坏了真菌细胞。值得注意的是，黑色素化是新孢梭菌的重要毒力决定因素，它不能保护隐球菌生物膜免受壳聚糖的侵害。在这项研究中用于评估真菌生物膜敏感性的壳聚糖浓度对人内皮细胞无毒。我们的结果表明，隐球菌生物膜对壳聚糖的治疗很敏感，这为预防或治疗留置医疗设备上的真菌生物膜提供了一种选择。

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