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首页> 外文期刊>Spine >Protein markers in cerebrospinal fluid in experimental nerve root injury. A study of slow-onset chronic compression effects or the biochemical effects of nucleus pulposus on sacral nerve roots.
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Protein markers in cerebrospinal fluid in experimental nerve root injury. A study of slow-onset chronic compression effects or the biochemical effects of nucleus pulposus on sacral nerve roots.

机译：实验性神经根损伤中脑脊液中的蛋白质标记物。关于slow神经迟缓慢性压迫作用或髓核生化作用的研究。

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STUDY DESIGN: Measurement of changes in cerebrospinal fluid concentrations of nerve tissue markers, total proteins, and immunoglobulin after compression of nerve root or application of nucleus pulposus in a pig model. OBJECTIVES: To assess whether compression or application of nucleus pulposus to spinal nerve roots may cause increased levels of cerebrospinal fluid markers of nerve tissue damage and total proteins, and whether synthesis of immunoglobulins may be induced in cerebrospinal fluid. SUMMARY OF BACKGROUND DATA: Previous studies have reported that there seems to be a relationship between elevated cerebrospinal fluid total protein concentrations, nerve tissue markers, clinical findings, and compression of the nerve root evaluated by radiologic changes in patients with sciatica. METHODS: Subjects included 41 pigs, including 5 control animals. In two groups of experimental animals (n = 7; n = 5), an ameroid constrictor was slid onto the S1 nerve root. In two other groups (n = 7; n = 5), nucleus pulposus harvested from the L2-L3 disc was applied to the S1 nerve root. Two sham animal groups (n = 7; n = 5) underwent the same laminectomy. Twenty-one pigs underwent reoperation after 1 week, and 15 pigs after 4 weeks. A syringe was used to remove 3 mL of cerebrospinal fluid at L4-L5. Concentrations of total proteins, the light subunit of the neurofilament protein, S-100 protein, neuron-specific enolase, and glial fibrillary acidic protein were measured, and the presence of oligoclonal bands (immunoglobulins) were assayed in cerebrospinal fluid. RESULTS: The pigs with compressed S1 nerve root had considerably higher neurofilament protein and total protein concentrations in their cerebrospinal fluid than the-control animals (P < 0.001 and P < 0.01, respectively) or the sham animals (P < 0.001 and P < 0.05) in the 1-week experiment. Nucleus pulposus did not induce a significant increase in concentrations of the different protein markers. The presence of oligoclonal bands in cerebrospinal fluid in the experimental groups did not differ between the control and sham animals. CONCLUSIONS: The neurofilament protein and total protein concentrations in cerebrospinal fluid may have diagnostic importance in cases wherein clinical findings are not clearly related to the radiologic changes and vice versa. These protein markers also may be useful tools in different experimental models.
机译：研究设计：在猪模型中，在压迫神经根或应用髓核后，测量神经组织标志物，总蛋白和免疫球蛋白的脑脊液浓度变化。目的：评估椎体神经根受压或施加髓核是否可能导致神经组织损伤和总蛋白的脑脊液标志物水平升高，以及是否可以在脑脊液中诱导免疫球蛋白的合成。背景数据摘要：先前的研究报道，坐骨神经痛患者的影像学变化评估了脑脊液总蛋白浓度升高，神经组织标志物，临床发现和神经根受压之间存在相关性。方法：受试者包括41头猪，包括5只对照动物。在两组实验动物（n = 7; n = 5）中，将类动物收缩器滑到S1神经根上。在另外两个组（n = 7; n = 5）中，将从L2-L3椎间盘收获的髓核应用于S1神经根。两个假动物组（n = 7; n = 5）进行了相同的椎板切除术。 1周后对21头猪进行了再手术，4周后对15头进行了再手术。使用注射器去除L4-L5处的3 mL脑脊液。测量总蛋白，神经丝蛋白轻亚基，S-100蛋白，神经元特异性烯醇化酶和神经胶质原纤维酸性蛋白的浓度，并在脑脊髓液中检测寡克隆带（免疫球蛋白）的存在。结果：S1神经根受压的猪的脑脊髓液中的神经丝蛋白和总蛋白浓度明显高于对照组（分别为P <0.001和P <0.01）或假动物（P <0.001和P <0.05） ）进行为期1周的实验。髓核没有引起不同蛋白质标志物浓度的显着增加。实验组和假手术组脑脊髓液中寡克隆带的存在没有差异。结论：在临床发现与放射学改变没有明显关系的情况下，脑脊液中神经丝蛋白和总蛋白的浓度可能具有诊断意义，反之亦然。这些蛋白质标记在不同的实验模型中也可能是有用的工具。

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