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首页> 外文期刊>Spine >Increase of 200-kDa neurofilament-immunoreactive afferents in the substantia gelatinosa in allodynic rats induced by compression of the dorsal root ganglion.
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Increase of 200-kDa neurofilament-immunoreactive afferents in the substantia gelatinosa in allodynic rats induced by compression of the dorsal root ganglion.

机译：背根神经节受压诱导的异常性大鼠明胶质中200 kDa神经丝免疫反应性传入细胞增加。

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STUDY DESIGN: An experimental study on mechanical allodynia, c-Fos expression, and 200-kDa-neurofilament immunoreactive (IR) afferent expression in the substantia gelatinosa related to compression of dorsal root ganglion (DRG). OBJECTIVES: To evaluate the presence of allodynia in DRG compression model and to demonstrate that the structural changes of spinal dorsal horn related to DRG compression. SUMMARY OF BACKGROUND DATA: A previous experimental report has demonstrated that the peripheral nerve injury may trigger some structural changes of the superficial spinal dorsal horn. These changes of the spinal dorsal horn were thought to be important for the modulation of pain sensations such as allodynia. METHODS: Sixty-eight male rats were used. The left L5 lamina was exposed and a drill hole was made in it. A stainless rod was placed close to the left L5 DRG through the drill hole. Behavioral testing with von Frey filament was performed. On day 28 after surgery, c-Fos expression in the spinal dorsal horn by non-noxious stimulation was examined. L5 spinal cord and bilateral L5 DRG specimens were stained with antibody for 200-kDa neurofilament (RT97). In addition, 2 or 3 spinal cord sections per rats were processed for immunoelectron microscopy. RESULTS: In the DRG compression group, the mechanical withdrawal threshold was decreased, c-Fos expression by non-noxious stimulation was observed in the spinal dorsal horn, and there were many RT97-IR afferents in the superficial spinal dorsal horn. Immunoelectron microscopic observations showed that RT97-IR terminals made synaptic contact with neurons in the superficial spinal dorsal horn. There were no significant differences in the distribution of RT97-IR neurons in DRG between compression and sham group. CONCLUSIONS: DRG compression induced allodynia and that RT97-IR afferents increased in the superficial dorsal horn of the spinal cord. The increase of RT97-IR afferents may be related to the mechanisms for the observed allodynia.
机译：研究设计：关于明胶质中机械性异常性疼痛，c-Fos表达和200 kDa神经丝免疫反应性（IR）传入表达的实验研究，其与背根神经节（DRG）的压缩有关。目的：评估DRG压迫模型中异常性疼痛的存在，并证明脊髓背角的结构变化与DRG压迫有关。背景数据摘要：先前的一项实验报告已经证明，周围神经损伤可能触发了浅表脊髓背角的某些结构变化。人们认为，脊髓背角的这些变化对于调节疼痛感（如异常性疼痛）很重要。方法：采用68只雄性大鼠。左L5椎板暴露，并在其中钻了一个孔。将不锈钢棒穿过钻孔放置在靠近左L5 DRG的位置。用von Frey灯丝进行了行为测试。手术后第28天，检查非有害刺激在脊髓背角的c-Fos表达。 L5脊髓和双侧L5 DRG标本用200 kDa神经丝抗体（RT97）染色。另外，对每只大鼠的2或3个脊髓切片进行免疫电子显微镜检查。结果：在DRG压迫组中，脊髓背角的机械退缩阈值降低，观察到无毒刺激引起的c-Fos表达，并且浅表背角中有许多RT97-IR传入体。免疫电子显微镜观察显示，RT97-IR末端与浅表脊髓背角的神经元发生突触接触。压缩组和假手术组之间DRG中RT97-IR神经元的分布没有显着差异。结论：DRG压迫可诱发异常性疼痛，且脊髓背浅角的RT97-IR传入量增加。 RT97-IR传入的增加可能与观察到的异常性疼痛的机制有关。

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