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首页> 外文期刊>Biomaterials >Enzymatically-crosslinked injectable hydrogels based on biomimetic dextran-hyaluronic acid conjugates for cartilage tissue engineering.
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Enzymatically-crosslinked injectable hydrogels based on biomimetic dextran-hyaluronic acid conjugates for cartilage tissue engineering.

机译：基于仿生葡聚糖-透明质酸缀合物的酶交联可注射水凝胶，用于软骨组织工程。

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摘要

Polysaccharide hybrids consisting of hyaluronic acid (HA) grafted with a dextran-tyramine conjugate (Dex-TA) were synthesized and investigated as injectable biomimetic hydrogels for cartilage tissue engineering. The design of these hybrids (denoted as HA-g-Dex-TA) is based on the molecular structure of proteoglycans present in the extracellular matrix of native cartilage. Hydrogels of HA-g-Dex-TA were rapidly formed within 2 min via enzymatic crosslinking of the tyramine residues in the presence of horseradish peroxidase and hydrogen peroxide. The gelation time, equilibrium swelling and storage modulus could be adjusted by varying the degree of substitution of tyramine residues and polymer concentration. Bovine chondrocytes incorporated in the HA-g-Dex-TA hydrogels remained viable, as shown by the Live-dead assay. Moreover, enhanced chondrocyte proliferation and matrix production were observed in the HA-g-Dex-TA hydrogels compared to Dex-TA hydrogels. These results suggest that HA-g-Dex-TA hydrogels have a high potential as injectable scaffolds for cartilage tissue engineering.
机译：合成了由透明质酸（HA）接枝葡聚糖-酪胺共轭物（Dex-TA）组成的多糖杂化物，并作为可注射的仿生水凝胶用于软骨组织工程研究。这些杂种的设计（称为HA-g-Dex-TA）是基于天然软骨细胞外基质中蛋白聚糖的分子结构而设计的。在辣根过氧化物酶和过氧化氢的存在下，通过酪胺残基的酶促交联，HA-g-Dex-TA的水凝胶在2分钟内迅速形成。可以通过改变酪胺残基的取代度和聚合物浓度来调节胶凝时间，平衡溶胀和储能模量。如活死分析所示，掺入HA-g-Dex-TA水凝胶的牛软骨细胞仍然具有活力。此外，与Dex-TA水凝胶相比，在HA-g-Dex-TA水凝胶中观察到软骨细胞增殖和基质产生增强。这些结果表明，HA-g-Dex-TA水凝胶作为用于软骨组织工程的可注射支架具有很高的潜力。

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