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首页> 外文期刊>Biomaterials >Protein adsorption, attachment, growth and activity of primary rat osteoblasts on polylactide membranes with defined surface characteristics
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Protein adsorption, attachment, growth and activity of primary rat osteoblasts on polylactide membranes with defined surface characteristics

机译：具有定义表面特征的大鼠原代成骨细胞在聚乳酸膜上的蛋白质吸附，附着，生长和活性

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摘要

The adsorption of proteins and growth and activity of primary rat osteoblasts cultured for 1, 2 and 3 weeks on nonporous and porous resorbable poly(L/DL-lactide) 80/20% membranes with defined surface characteristics were investigated. The growth and activity of cells were estimated from the measurements of DNA, alkaline phosphatase activity and the total amount of protein in the cell lysate. The cell morphology was assessed from scanning electron microscopy and rhodamine staining. The protein adsorption to the membrane surface was assessed from the amide I peak at 1640-1660 cm(-1) and the amide II peak at 1540-1560 cm(-1) in the attenuated total reflection infrared spectra. The relative amount of proteins adsorbed on the nonporous and porous membranes was comparable. The cells growing on the nonporous and porous membranes maintained the phenotype and revealed morphology typical for osteoblasts. The mineralized noduli were larger in size oil the porous membranes. The number of cells, the amount of DNA, the alkaline phosphatase activity, and the total amount of protein increased with time of the experiment and were higher for the porous membranes than for the nonporous ones. (C) 2003 Elsevier Ltd. All rights reserved. [References: 69]
机译：研究了在具有确定的表面特性的无孔和多孔可吸收聚（L / DL-丙交酯）80/20％膜上培养1、2和3周的原代大鼠成骨细胞的蛋白质吸附以及生长和活性。细胞的生长和活性通过DNA，碱性磷酸酶活性和细胞裂解物中蛋白质总量的测量值进行估算。通过扫描电子显微镜和若丹明染色评估细胞形态。在衰减的全反射红外光谱中，从酰胺I峰在1640-1660 cm（-1）和酰胺II峰在1540-1560 cm（-1）处评估膜表面的蛋白质吸附。吸附在无孔和多孔膜上的蛋白质的相对量是可比较的。在无孔和多孔膜上生长的细胞保持表型并显示成骨细胞典型的形态。多孔膜上的矿化结节尺寸较大。细胞数量，DNA量，碱性磷酸酶活性和蛋白质总量随实验时间的增加而增加，并且多孔膜要高于无孔膜。 （C）2003 Elsevier Ltd.保留所有权利。 [参考：69]

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