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首页> 外文期刊>Biomaterials >BIOCOMPATIBILITY STUDIES ON GLASS IONOMER CEMENTS BY PRIMARY CULTURES OF HUMAN OSTEOBLASTS
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BIOCOMPATIBILITY STUDIES ON GLASS IONOMER CEMENTS BY PRIMARY CULTURES OF HUMAN OSTEOBLASTS

机译：人类成骨细胞原始培养物对玻璃离子型水泥的生物相容性研究

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Glass ionomer cements (GICs) are materials largely employed in the dental field that have been considered recently as cements in orthopaedic surgery for their proven osteogenic features. The aim of this study was to compare the response of cultured human osteoblastic cells to a number of commercial glass ionomer cements in order to provide indications useful for the further development of formulations that have potential for use as cements or implants in repair and replacement of bone tissue. The GICs tested were: Ketac-Fil Aplicap, lonocem lonocap 1,0, GC Fuji II, GC Fuji II LC and Vitremer 3M. Several features such as plating efficiency, adhesion and morphology of the cells were studied, as well as the only specific biochemical parameter of osteoblastic phonotype, namely osteocalcin production. In addition, the colonisation of materials by osteoblastic cells was verified by means of scanning electron microscopy. Altogether, the results obtained indicate that four of the five glass ionomer cements tested are biocompatible, showing vital cells adhering to the materials, proliferating and expressing the biochemical markers of osteoblastic phenotype, whereas Vitremer 3M, although currently employed in the dental field, exhibits a great cytotoxicity toward the cells. The adverse reaction of this GIC can be attributed to the leaching of at least two components of the polyacidic phase evidenced by protonic magnetic resonance analysis (PMR), namely 2-hydroxyethyl methacrylate (HEMA), and an un identified acidic species. The addition of pure HEMA at the same concentrations found by means of PMR to cultures of osteoblastic cells resulted in a complete cell death. Our results also show that in vitro methods employing primary cultures of human cells specific to the implant sites of prostheses are appropriate and suitable tools for evaluating biocompatibility of materials. Furthermore, this kind of approach can provide indications useful in the design of novel materials as well as in improving the characteristics of the formulations already available. (C) 1996 Elsevier Science Limited [References: 26]
机译：玻璃离聚物粘固剂（GIC）是牙科领域中广泛使用的材料，最近由于其已证实的成骨特性而被认为是骨科手术中的粘固剂。这项研究的目的是比较培养的人成骨细胞对多种市售玻璃离子水门汀的反应，从而为进一步开发可作为骨水泥或植入物用于修复和置换骨的配方提供有用的适应症。组织。测试的GIC为：Ketac-Fil Aplicap，lonocem lonocap 1,0，GC Fuji II，GC Fuji II LC和Vitremer 3M。研究了诸如铺板效率，细胞粘附和形态的几个特征，以及成骨细胞表型的唯一特定生化参数，即骨钙素的产生。此外，通过扫描电子显微镜证实成骨细胞对材料的定殖。总体而言，获得的结果表明，所测试的五种玻璃离聚物水泥中有四种具有生物相容性，显示出重要细胞粘附在材料上，能够增殖并表达成骨细胞表型的生化标记，而Vitremer 3M尽管目前在牙科领域使用，但仍具有一定的生物相容性。对细胞有很大的细胞毒性。该GIC的不良反应可归因于质子磁共振分析（PMR）所证明的多酸性相中至少两种组分的浸出，即甲基丙烯酸2-羟乙酯（HEMA）和未鉴定的酸性物质。在成骨细胞培养物中加入与PMR相同浓度的纯HEMA，导致细胞完全死亡。我们的结果还表明，采用特定于假体植入部位的人类细胞原代培养的体外方法是评估材料生物相容性的合适方法。此外，这种方法可以提供适用于新型材料设计以及改善现有制剂特性的适应症。 （C）1996 Elsevier Science Limited [参考：26]

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